en عمومى General پژوهشي Original article Background and Aims: This study was the trends of Hepatitis B infection among Iranian blood donors which was analyzed in a period of 10 years. Materials and Methods: In a period of 10 years, from 2001 through 2010, a total of 16,264,830 donations from 30 regional and 35 local blood services were screened. All blood samples were tested for Hepatitis B surface antigen (HBs Ag) by commercial available kits. Sistan-Baluchestan (S&B) province representing a high prevalence and Fars province was a low prevalence area. For assessing frequency of infection, the prevalence of HBS Ag per 100 000 donations and 95% confidential intervals (95% CIs) was calculated. Statistical analysis was conducted using chi-square test and considered significant if P value was <0.05. Results: The prevalence rates of HBs Ag dramatically declined from 1.23% in 2001 to 0.25% in 2010 in Iran. In S&B province HBs Ag prevalence decreased from 3.29% in 2001 to 0.66% in 2010 and in Fars province, the rate of HBs Ag decreased from 0.82% in 2001 to 0.12% in 2010. In this period, the number of donation progressively increased from 1361321 donation in 2001 to 1889851 donation in 2010, P v <0.00001. The number of volunteer donations increased from 92% in 2001 to 100% in 2010. Conclusion: The findings indicated that an appropriate implementation of Iranian Blood Transfusion Organization (IBTO) programs such as the selection of blood donors/ donor recruitment, increasing non-remunerated repeat donors, routine screening for blood borne viruses, replacement donation exclusion, and implementation of automation are being performed. Hepatitis B virus, Blood Donation, Prevalence, Iranian Blood Transfusion Organization (IBTO) 8 12 http://journal.isv.org.ir/browse.php?a_code=A-10-1-73&slc_lang=en&sid=1 M Mahmoodian-Shooshtari 10031947532846003504 10031947532846003504 Yes Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran Z Sharifi 10031947532846003505 10031947532846003505 No Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran SE Mousavi 10031947532846003506 10031947532846003506 No Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran SA Ahmadi-Teymourloyee 10031947532846003507 10031947532846003507 No Department of Health Services Management, School of Health Management and Information Sciences, Tehran University of Medical Sciences