:: Volume 7, Issue 4 (11-2013) ::
Iran J Virol 2013, 7(4): 7-13 Back to browse issues page
Development a New Human Skin Continuous Cell Line Sensitive to Mumps Virus
N Mahmudi-Gharoeie, A Mohammadi , B Saffar, F Esna-Ashari, A Foroghi, B Alirezaee, R Ghorbani, ZA Sedigh
Human Viral Vaccine Department, Razi Vaccine and Research Institute, Alborz Karaj, Iran
Abstract:   (3690 Views)
Background and Aims: During the last years a new Foreskin cell line strain (RFSC) has been isolated, characterized and stored in a representative cell bank stock in liquid nitrogen as a part of HCB (Human Cell Bank) development program in Razi institute. Materials and Methods: The cell population was serially passaged for mass production purpose based on WHO schedule .The controls on pool samples were carried out upon our sampling method and international recommendations. Results: The data concerning parameters as well as the result of compulsory controls on Kario logy oncogenicity, cross contaminations, species specificity, no oncogenicity, and mycoplasma free of a human cell line that contained heteroploid chromosomal characterizations are performed. Conclusion: Kariological examination found an obvious discrepancy in the frequency of aberrations due to high passage level that produced a heteroploid continuous cell line. Virus susceptibility shows RFSC-1 more sensitive to RS#12 Mumps virus strain. This cell line is completely sterile and has no any cross contamination to other pathogen and can be used for production and control of Mumps vaccine.
Keywords: Primary culture, Cell Characterization, Human Cell Bank, RFSC-1
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Type of Study: Original article | Subject: General
Received: 2015/08/21 | Accepted: 2015/08/21 | Published: 2015/08/21



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Volume 7, Issue 4 (11-2013) Back to browse issues page