:: Volume 8, Issue 1 (2-2014) ::
Iran J Virol 2014, 8(1): 33-34 Back to browse issues page
Putative RFLP Analysis between HSVd-sb and Closely Related Variant
SAA Bagherian
Department of Horticultural Sciences, College of Agriculture, Jahrom University, Jahrom, Iran.
Abstract:   (2328 Views)

Sweet lime split bark is a disorder of increasing importance in Fars province. Previously we showed that a new variant of hop stunt viroid (HSVd-sb) was related to the disorder. It had homology with a noncachexia variant of hop stunt viroid (CVd-IIa-117) which causes cracking the bark of trifoliate orange rootstocks. In this study, showed that M.NgoBIX, BglI and BsrI restriction enzymes can cut HSVd-sb according to putative RFLP analysis using Vector NTI program (version 9.0.0) database but these restriction enzymes can’t cut CVd-IIa-117. In other hand some restriction enzymes can cut CVd-IIa-117 but can’t cut HSVd-sb. Therefore these two variants can be recognized by these restriction enzymes.

Keywords: HSVd-sb, CVd-IIa-117, Restriction Fragment Length Polymorphism analysis
Full-Text [PDF 58 kb]   (434 Downloads)    
Type of Study: Original article | Subject: General
Received: 2015/12/18 | Accepted: 2015/12/18 | Published: 2015/12/18



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Volume 8, Issue 1 (2-2014) Back to browse issues page