Putative RFLP Analysis between HSVd-sb and Closely Related Variant
|
SAA Bagherian * |
Department of Horticultural Sciences, College of Agriculture, Jahrom University, Jahrom, Iran. |
|
Abstract: (4107 Views) |
Sweet lime split bark is a disorder of increasing importance in Fars province. Previously we showed that a new variant of hop stunt viroid (HSVd-sb) was related to the disorder. It had homology with a noncachexia variant of hop stunt viroid (CVd-IIa-117) which causes cracking the bark of trifoliate orange rootstocks. In this study, showed that M.NgoBIX, BglI and BsrI restriction enzymes can cut HSVd-sb according to putative RFLP analysis using Vector NTI program (version 9.0.0) database but these restriction enzymes can’t cut CVd-IIa-117. In other hand some restriction enzymes can cut CVd-IIa-117 but can’t cut HSVd-sb. Therefore these two variants can be recognized by these restriction enzymes.
|
|
Keywords: HSVd-sb, CVd-IIa-117, Restriction Fragment Length Polymorphism analysis |
|
Full-Text [PDF 58 kb]
(1914 Downloads)
|
Type of Study: Original article |
Subject:
General Received: 2015/12/18 | Accepted: 2015/12/18 | Published: 2015/12/18
|
|
|
|