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جلد 9 شماره 3 صفحات 24-31 برگشت به فهرست نسخه ها
Novel variants of citrus exocortis viroid from southern of Iran
SAA Bagherian
Department of Horticultural sciences, College of Agriculture, Jahrom University, Jahrom, Iran
چکیده:   (2777 مشاهده)

Background and Aims: Citrus exocortis disease is naturally limited to citrus plants and causes a serious disease on sensitive rootstocks such as trifoliate orange. The aim of this study was to sample new variants of citrus exocortis viroid from Fars trees with yellowing and suberization symptoms and comparison with other isolates of this viroid.

Materials and Methods: A number of symptomatic or symptomless citrus trees were sampled and studied for viroid detection. After RNA extraction, reverse transcription-polymerase chain reaction was conducted and the full length genome of viroids were cloned and sequenced. Three CEVd isolates from Fars were selected and used for comparison with four CEVd isolates from Mazandaran and a number of other sequences from GenBank.

Results: It was shown that on the basis of homology, three CEVd isolates from Mazandaran and all three CEVd isolates from Fars clustered into one group while one of the Mazandaran sequences fell apart.

Conclusions: Therefore we proposed that Iranian CEVd isolates except one isolate from north have the same origin.

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نوع مطالعه: پژوهشي | موضوع مقاله: عمومى
دریافت: 1395/8/23 | پذیرش: 1395/12/2 | انتشار: 1395/12/2
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Novel variants of citrus exocortis viroid from southern of Iran. Iran J Virol. 2015; 9 (3) :24-31
URL: http://journal.isv.org.ir/article-1-248-fa.html

Bagherian SAA. Novel variants of citrus exocortis viroid from southern of Iran. مجله ویروس شناسی ایران. 1394; 9 (3) :24-31

URL: http://journal.isv.org.ir/article-1-248-fa.html



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دوره 9، شماره 3 - ( 5-1394 ) برگشت به فهرست نسخه ها
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