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جلد 9 شماره 4 صفحات 18-23 برگشت به فهرست نسخه ها
Virus shedding after H9N2 strain challenge of SPF Chickens immunized by an experimental Nano-Adjuvant and commercial oil emulsion avian influenza (H9N2) vaccines
چکیده:   (3104 مشاهده)

Abstract

Background and Aims: Vaccination of poultry has a major impact on the prevention and control of avian influenza viruses. Nanobiotechnology techniques provide a new approach for improvement of influenza vaccine efficacy. In this study, efficacy of an inactivated nano-adjuvant vaccine developed based on an endemic H9N2 virus was evaluated in SPF chickens.

Materials and Methods: In each three trial 40 specific pathogen free (SPF) white Leghorn chickens were used in four groups. Chickens from treatment groups (n= 10) via subcutaneous route received a single dose of the nano-adjuvant or the oil emulsion Razi® H9N2 vaccines. Chickens in the control group C received antigen only. All the birds were challenged with H9N2 strain on day 21 post-vaccination. Cloacal and tracheal swabs were taken at 1-10 days post-challenge and viral shedding was examined using inoculation of SPF embryonated eggs.

Results: Both vaccinated SPF chicken groups induced complete protection against clinical signs. Viral shedding in the nano-adjuvant H9N2 vaccinated chickens was completely blocked after challenge with a homologous H9N2 virus. Statistical analysis based on the protection effects of the chickens immunized with nano-adjuvant and the Razi® H9N2 vaccine showed no significant difference, but there was a significant difference to un-vaccinated group.

Conclusions: The results of this study indicated that the nano-adjuvant vaccine was efficacious in protection of SPF chickens over H9N2 infection. Further field experiments are needed to demonstrate the efficacy of the vaccines under field conditions.

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نوع مطالعه: پژوهشي | موضوع مقاله: تخصصي
دریافت: 1395/8/23 | پذیرش: 1395/12/7 | انتشار: 1395/12/7
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Mojahedi Z, Vasfi Marandi M, Aghaipour K, Mahdavi H, Ppourbakhsh S A, Anissian A. Virus shedding after H9N2 strain challenge of SPF Chickens immunized by an experimental Nano-Adjuvant and commercial oil emulsion avian influenza (H9N2) vaccines. Iran J Virol. 2015; 9 (4) :18-23
URL: http://journal.isv.org.ir/article-1-249-fa.html

Virus shedding after H9N2 strain challenge of SPF Chickens immunized by an experimental Nano-Adjuvant and commercial oil emulsion avian influenza (H9N2) vaccines. مجله ویروس شناسی ایران. 1394; 9 (4) :18-23

URL: http://journal.isv.org.ir/article-1-249-fa.html



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دوره 9، شماره 4 - ( 5-1394 ) برگشت به فهرست نسخه ها
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