Cytomegalovirus is the most important pathogen affecting transplant recipients and causing significant morbidity and morbidity. The prevalence of CMV  infections in transplant recipient varies from country to country.  The aim of this study was to determine the prognostic value of PCR assay for CMV  detection in patients before Keratoplasty.  A total of 23 patient samples enrolled in this study from March 2008 to Feb 2010.  Based on the result the over all incidence of CMV  infection was 39.1 percent (9 positive sample) in PCR assay.  In this study, the plasma PCR assay proved to be sensitive and specific in order to detect CMV  infections.