Preparation of a pcDNA3 Plasmid for Hemagglutinin-Neuraminidase Gene of Human Respirovirus 3 (HPIV-3)
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D Behzadpour * , R Alizadeh , T Bamdad , M Daliri , A Seidavi |
Department of Virology, Rasht Branch, Islamic Azad University, Rasht, IR Iran. |
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Abstract: (2903 Views) |
Background and Aims: Human respirovirus 3 is the second most common agent for respiratory disease in children under five years old. Near 68% of respiratory tract infections belong to this type. However, there is no established vaccine for this virus. The aim of this study was to synthesize a DNA plasmid for HN gene of human respirovirus 3 (HPIV-3) as a first step for the preparation of a vaccine.
Materials and Methods: The HN gene was synthesized and cloned into pcDNA3 plasmid. The plasmids were transformed into COS-7 cell lines. To evaluate the expressed HN protein, the western blotting assay was applied.
Results: The HN protein 36 KD was detected on nitrocellulose membrane using anti-His-tag antibodies.
Conclusions: We prepared a DNA plasmid containing HN gene of human respirovirus 3 to be used for the future studies towards making an effective vaccine. Among hospitalized Iranian children, one-month to five-year, human respirovirus 3 was most frequently observed in respiratory tract infection. |
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Keywords: DNA plasmid, Human respirovirus, Human parainfluenza, HN gene, cell culture |
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Full-Text [PDF 329 kb]
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Type of Study: Original article |
Subject:
Special Received: 2018/12/15 | Accepted: 2019/01/26 | Published: 2019/01/26
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