:: Volume 13, Issue 2 (12-2019) ::
Iran J Virol 2019, 13(2): 0-0 Back to browse issues page
Assessment of Foot and Mouth Virus Subtype O2016 Genetic Alterations During Successive Passages in BHK Monolayer
Homayoon Mahravani , Homayoon Mahravani
National FMD research education ference laboratory,Agricultural research education and extention organization (AREEO)
Abstract:   (203 Views)
Abstract : Foot and Mouth Disease is one of  the important live stocks contagious viral disease caused by Aphtovirus genus ,  that is belong to family RNA virus  picornaviride. The important characteristic of FMD virus is high mutation that give rise to diversity of Antigen in surface of Neutralizing proteins. For this reason FMD virus have 7 distinct serotype and many subtype. Vaccination is one of the methods for control disease in order to different type and subtype  of FMD virus , the effective vaccine should have virus that closed relationship with field virus and also many passage on cell culture may cause mutation on viral genome . The rate of genetic changes of FMD virus type O2016 during of 70 passages in BHK monolayer  cell is the main reasone of this paper .  After determine the type of virus in epithelium sample by ELISA , the virus inoculated in BHK monolayer cell for 70 consequence passage . harvested viruses in 10 selected passage (P1,P10 , P20,P30,P40,P50,P60,P70) is done by RT-PCR , ELISA , titration , real time PCR, Double dimension virus neutralization test for  immunological relationship value (r value) and Nucleotide sequencing of 1D segment  of viral genome . harvested FMD virus type O2016  From passage 1 until 70  be constant and no significant change neither in Sequencing nor in r value .
Keywords: Key words : Foot and Mouth Diease, Genome Sequence, Serotype, Antigenic Variation, Mutation .
Type of Study: Original article | Subject: Special
Received: 2020/01/17 | Accepted: 2020/04/15 | Published: 2020/04/15

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Volume 13, Issue 2 (12-2019) Back to browse issues page