[صفحه اصلی ]   [Archive] [ English ]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 5، شماره 3 - ( 5-1390 ) ::
جلد 5 شماره 3 صفحات 7-11 برگشت به فهرست نسخه ها
Set up of Genotyping Test for Detection of HIV-1 Drug Resistance
چکیده:   (2297 مشاهده)
Background and Aims: Genotyping assay has been accepted as a guidance in the therapeutic management of Human Immunodeficiency virus 1 (HIV-1). But, it is not commonly used in our country due to its high running cost. The aim of this study is evaluate an in-house genotyping resistance test (GRT) for HIV-1. Methods: HIV-1 RNA of 20 samples were extracted from plasma and RT Nested- PCR was performed and the final products were sequenced. Stanford HIV Sequence Database was used for genotyping and interpretation of resistance data. Results: Subtype A was the dominant viral subtype in these patients. Also the results of drug resistance interpretation showed that drug-naïve patients are susceptible to drugs and for patients taking the drugs 66.6% susceptible for AZT, 50% high- level resistance for 3TC, 66.6% low- level resistance for ABC, 66.6% susceptible for TDF, 50% high- level resistance for NVP and 50% high- level resistance for EFV. Conclusion: Our method is able to amplify and sequence HIV-RNA from plasma samples from a random selection of patients encompassing different subtypes. The results of this study may have important consequences for survey and clinical management of patients with AIDS in Iran.
متن کامل [PDF 270 kb]   (475 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: عمومى
دریافت: ۱۳۹۳/۸/۱۶ | پذیرش: ۱۳۹۳/۸/۱۶ | انتشار: ۱۳۹۳/۸/۱۶
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA



XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Baesi K, Saberfar E, Haji-Abdolbaghi M, Goodarzi Z. Set up of Genotyping Test for Detection of HIV-1 Drug Resistance. Iran J Virol. 2011; 5 (3) :7-11
URL: http://journal.isv.org.ir/article-1-72-fa.html

Set up of Genotyping Test for Detection of HIV-1 Drug Resistance. مجله ویروس شناسی ایران. 1390; 5 (3) :7-11

URL: http://journal.isv.org.ir/article-1-72-fa.html



دوره 5، شماره 3 - ( 5-1390 ) برگشت به فهرست نسخه ها
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 3961