%0 Journal Article %A Zamani, S %A Fotouhi Chahouki, F %A Mehrbod, P %A Sadeghi Neshat, S %A Farahmand, B %T Production and Evaluation of Polyclonal Antibody Against Influenza A Virus Matrix 2 Conserved Protein for Research and Diagnosis Purposes %J Iranian Journal of Virology %V 11 %N 4 %U http://journal.isv.org.ir/article-1-335-en.html %R %D 2017 %K ELISA, Influenza Virus, M2 protein, polyclonal antibody, RID., %X Background and Aims: The aim of this study was to produce and purify the Polyclonal antibody (pAb) against Matrix protein 2 (M2) with reasonable efficiency. Matrix protein 2 is one of the most conserved proteins of the influenza A virus which acts as ion channel. Polyclonal antibodyproduced against Matrix protein 2 is used in vaccine research, passive immunization and qualitative/quantitative analysis methods. Materials and Methods: Recombinant M2 protein was produced in E.coli. Purified protein with Freund’s adjuvants (Complete and Incomplete) was injected into two New-Zealand white male rabbits. The polyclonal antisera of rabbits were evaluated by RID, immunoblotting and ELISA. The IgG was purified using DAEA-cellulose column chromatography. Finally, the quality and properties of purified IgG were evaluated using SDS-PAGE and ELISA. Results: The RID and immunoblotting results showed that the produced anti-M2 antibody was able to recognize M2 recombinant protein epitopes. The ELISA results confirmed anti-M2 pAb reached reasonable titers after three injections. IgG against M2 was purified with suitable concentration. The Purified polyclonal IgG-M2 was evaluated using ELISA and the results showed IgG-M2 reacted with the antigen up to 1:32000. Conclusions: The data showed that recombinant M2 protein was able to stimulate immune response to produce antibody at satisfactory level. %> http://journal.isv.org.ir/article-1-335-en.pdf %P 31-38 %& 31 %! %9 Original article %L A-10-214-1 %+ Department of Influenza and other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran %G eng %@ 1735-5680 %[ 2017