Iranian Journal of Virology
مجله ویروس شناسی ایران
Iran J Virol
Medical Sciences
http://journal.isv.org.ir
1
admin
1735-5680
2588-5030
10.21859/isv
en
jalali
1392
6
1
gregorian
2013
9
1
7
3
online
1
fulltext
en
Phylogenetic Study Based on the Gene of Attachment Protein (G) Avian Metapneumovirus from Broiler Breeder farm in Iran, 2013
عمومى
General
پژوهشي
Original article
Background and Aims: Avian metapneumovirus (aMPV) is RNA virus responsible for upper respiratory disease in poultry which is usually accompanied by secondary bacterial infections. In Iran, some molecular and serological studies indicated that aMPV infection occurred in commercial poultry industry. Materials and Methods: In this study aMPV was molecularly identified and characterized in an affected broiler breeder flock. The well-vaccinated flock was suffering from a disease with clinical signs and a drop in egg production. After RNA extraction from tracheal and oropharyngeal samples, the presence of viral RNA was demonstrated by reverse transcription-polymerase chain reaction using primers specific to the attachment glycoprotein(G) gene. Positive samples were sequenced and phylogenetic tree based on sequences results was drawn. Based on sequences analysis, field strain of aMPV was recovered from the flock. Results: Phylogenetic analysis showed that the virus (PCRLAB/HG2013) is located in B subtype cluster and is closely related to the aMPV strain that was previously detected in Iran (PCRLAB/HG2010) and other countries (Nigeria, Japan and Brazil). Conclusion: The results showed that field strain of aMPV has the potential to cause disease and economic losses in breeder broiler especially during egg production period. It is also concluded that vaccination against aMPV may not provide full protection in field situation and other control measurements including biosecurity should be taken.
Avian metapneumovirus, Iran, Phylogenetic analysis, Broiler Breeder
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11
http://journal.isv.org.ir/browse.php?a_code=A-10-40-3&slc_lang=en&sid=1
Arash
Ghalyanchi-Langeroudi
10031947532846003384
10031947532846003384
No
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Hadi
Haghbin-Nazarpak
10031947532846003385
10031947532846003385
No
Department of Clinical Sciences, Collage of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran
Hossein
Hosseini
10031947532846003386
10031947532846003386
Yes
Department of Clinical Sciences, Collage of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran