en عمومى General پژوهشي Original article Background and Aims: Adenovector gene transfer induces inflammatory response that finally leads to vector removal from delivered site. The effect of hydrodynamic pre-injection on Adenovector mediated liver inflammation remained elusive. Materials and Methods: Different mice groups were pre-treated by hydrodynamic saline, dexamethasone or nothing prior to Adeno-Luciferase administration. Their samples were collected in 18 hours later and serum IL-1&beta; level as a marker of inflammation was quantified. Also, liver histological assessment was performed to score the inflammatory grade. Results and Conclusion: In spite of dexamethasone group, both hydrodynamic/Ad-Luc and Ad-Luc receiving groups induced the expression of IL-1&beta; cytokine (p<0.05). The pathological views also indicated more inflammation and necrosis inside the different zones of liver tissue in these two adenovector injected groups. These results emphasized that Adenovector served as a suitable inflammatory model and hydrodynamic injection enhances this kind of inflammation. Hydrodynamic injection, Adenoviral vector, Inflammation 22 26 http://journal.isv.org.ir/browse.php?a_code=A-10-40-6&slc_lang=en&sid=1 Fatemeh Rezaei 10031947532846003396 10031947532846003396 No GastroenteroHepatology Research center, Shiraz University of Medical Sciences, Shiraz, Iran Seyed Younes Hosseini 10031947532846003397 10031947532846003397 Yes GastroenteroHepatology Research center, Shiraz University of Medical Sciences, Shiraz, Iran Ameneh Pakniat 10031947532846003398 10031947532846003398 No Shiraz Transplant Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran Bit Geramizadeh 10031947532846003399 10031947532846003399 No Shiraz Transplant Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran Mohammadreza Fattahi 10031947532846003400 10031947532846003400 No GastroenteroHepatology Narges Nejabat 10031947532846003401 10031947532846003401 No GastroenteroHepatology Research center, Shiraz University of Medical Sciences, Shiraz, Iran Farhad Koohpeyma 10031947532846003402 10031947532846003402 No Endocrine and metabolism research center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran