en تخصصي Special پژوهشي Original article <p>Foot and Mouth Disease (FMD) is highly contagious disease among cloven-hoofed animals. FMD virus has structural and non-structural proteins. Vp1 is the most immunogenic structural peptides of FMD virus, applied for major vaccine studies. Objective: Construction of Pet28-VP1 cassette for FMD virus type O/IRN/2010 and expression VP1 peptide as the most immunogenic antigen was the aim of this study. Methods: The FMD virus type O/IRN/2010 was isolated from cattle in Qom, Iran and propagated on IBRS2 and BHK21 cell lines. The VP1 gene was amplified using the specific primer pair and RT-PCR technique. The purified PCR product was sub-cloned into the unique BamHI and Xho1 cloning sites to construct the PTZ57R/T -VP1 cassette. The DH5&alpha strain was transformed with this cassette. The digested VP1 gene was cloned in the digested Pet28 and confirmed using double digestion. Then the Pet28-VP1 construction was transformed in BL21 strain. Results: Expression of VP1 peptide was evaluated by IPTG induction and SDS-PAGE and confirmed by Guinea pig specific polyclonal antibody against FMD virus type O and conjugated rabbit anti Guinea pig antibody- HRP. Also neutralizing antisera titre was protective for vaccinated animals by recombinant VP1 protein. Conclusion: Since the isolation of new FMD virus strains in different geographical locations and expression of VP1 peptide can be used in emergency and control settings as a recombinant vaccine in the same area. Therefore the Pet28-VP1 cassette was constructed in this study is a good candidate for preparation of peptide vaccine against FMD virus type O/IRN/2010 in the next future.</p> Foot and Mouth Disease Virus, VP1 protein, Peptide vaccine, Immune Response 12 17 http://journal.isv.org.ir/browse.php?a_code=A-10-66-1&slc_lang=en&sid=1 Farahnaz Motamedi-Sedeh fmotamedi@nrcam.org 10031947532846003285 10031947532846003285 Yes Nuclear Science and Technology Research Institute, Veterinary and animal Science department Homayoon Mahravani mahravani2010@gmail.com 10031947532846003286 10031947532846003286 No Razi vaccine and Serum Research Institute Hoorieh Soleimanjahi soleim_h@modares.ac 10031947532846003287 10031947532846003287 No Tarbiat Modares University, Faculty of Medical Science Lila Asadpour l.asadpour@yahoo.com 10031947532846003288 10031947532846003288 No Islamic Azad University, Gylan Branch, Science faculty, biological Group Kamalodin Shafaei kshafaie@nrcam.org 10031947532846003289 10031947532846003289 No Nuclear Science and Technology Research Institute, Veterinary and animal Science Department