Iranian Journal of Virology
مجله ویروس شناسی ایران
Iran J Virol
Medical Sciences
http://journal.isv.org.ir
1
admin
1735-5680
2588-5030
10.21859/isv
en
jalali
1394
5
1
gregorian
2015
8
1
9
3
online
1
fulltext
en
New Hosts and Comparison of Biological and Molecular Characteristics of Fig, Mulberry, and Citrus Isolates of Hop Stunt Viroid in Iran
تخصصي
Special
پژوهشي
Original article
<div>
<p><strong>Abstract</strong></p>
<p><strong>Background and Aims:</strong> Hop stunt viroid (HSVd, genus Hostuviroid, family Pospiviroidae) has a wide host range among trees and herbaceous plants. The objectives of this study were to compare biological and physical characteristics of three isolates of HSVd from mulberry, fig and citrus and search for other hosts of this viroid in Iran.</p>
<p><strong>Materials and Methods:</strong> Plant samples were collected from Yazd, Isfahan, Fars and Tehran provinces and examined for their possible infection by hop stunt viroid (HSVd) using reverse transcription- polymerase chain reaction (RT-PCR). The experimental host range of the viroid was determined by injection of infected sap into greenhouse grown seedlings and subsequent assay of inoculated plants by RT-PCR. Fig, mulberry and citrus isolates of the viroid were compared with respect to host range and molecular characteristics. Nucleic acids were isolated from plant tissues by CTAB method and subjected to RT-PCR using HSVd specific primers. The PCR products were sequenced and the most stable secondary structures for the three mentioned isolates were predicted and compared. </p>
<p><strong>Results:</strong> Among 120 samples collected from different regions and hosts, 13 samples from mulberry, fig, apple, quince, apricot, peach and citrus were infected by HSVd. The mulberry samples showed vein clearing and leaf deformation symptoms while usually no specific symptoms were observed in the fig samples. Apple, quince, apricot, peach and citrus samples were also symptomless hosts. In greenhouse tests, mulberry isolate induced more severe symptoms. Comparison of primary and secondary structures of the viroid isolates showed a closer similarity between the fig and mulberry isolates while the latter were less similar to citrus isolate.</p>
<p><strong>Conclusions: </strong>Sequence variation and structural differences were observed amon the isolates studied. Most differences of these isolates were in the pathogenicity, variable and terminal right regions which may determine the type andseverity of symptoms. Many trees are infected without showing apparent symptoms.</p>
</div>
hop stunt viroid, fig, citrus, mulberry
15
23
http://journal.isv.org.ir/browse.php?a_code=A-10-46-1&slc_lang=en&sid=1
Zahra
Jahanshahi
Z
Jahanshahi
zahrajahanshahi87@yahoo.com
10031947532846003164
10031947532846003164
No
Shiraz University
Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran
Keramat
Izadpanah
K
Izadpanah
izadpana@shirazu.ac.ir
10031947532846003165
10031947532846003165
Yes
Shiraz University
Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran
Alireza
Afsharifar
AR
Afsharifar
afshari@shirazu.ac.ir
10031947532846003166
10031947532846003166
No
Shiraz University
Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran
Seyed Ali Akbar
Behjatnia
SAA
Behjatnia
behjatni@shirazu.ac.ir
10031947532846003167
10031947532846003167
No
Shiraz University
Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran