Iranian Journal of Virology
مجله ویروس شناسی ایران
Iran J Virol
Medical Sciences
http://journal.isv.org.ir
1
admin
1735-5680
2588-5030
10.21859/isv
en
jalali
1392
11
1
gregorian
2014
2
1
8
4
online
1
fulltext
en
Clone Purification, Characterization and Standardization of LaSota Strain for Developing a Live Vaccine against Newcastle Disease Virus
تخصصي
Special
پژوهشي
Original article
<p dir="ltr" style="text-align: justify;">Newcastle disease (ND) is one of the most serious illnesses of chickens. Live vaccines are widely used to prevent chicken from the disease all over the world. To access the effective and potentiate ND vaccine, a homogenous subpopulation from LaSota strain was selected following cultivation of the virus on primary chicken embryofibroblast (CEF) cells. Pathogenicity indices and molecular characterization of a several plaques at 3rd passage were analyzed and then the selected clone was candidate for vaccine development. ND vaccine was prepared according to the standard protocols. The immunogenicity of the live vaccine was examined in specific pathogen free (SPF) and commercial chickens. The geometric mean hemagglutination-inhibition (HI) titers induced in chickens vaccinated with the cloned vaccine were not significantly differ than those induced in chickens vaccinated with the similar ND clone vaccine. Efficacy of the ND vaccine was estimated against the virus challenge. The result indicates the ability of the cloned vaccine to confer a complete protection against NDV.</p>
Newcastle disease virus, LaSota, clone purification, live vaccine
12
19
http://journal.isv.org.ir/browse.php?a_code=A-10-83-1&slc_lang=en&sid=1
MM
Ebrahimi
10031947532846003252
10031947532846003252
Yes
Department of Poutry Vaccines Research and Production, Razi Vaccine & Serum Research Institute, Karaj, Iran
S
Shahsavandi
10031947532846003253
10031947532846003253
No
Department of Poutry Vaccines Research and Production, Razi Vaccine & Serum Research Institute, Karaj, Iran
H
Famil-Ghadakchi
10031947532846003254
10031947532846003254
No
Razi Vaccine & Serum Research Institute, Nourthwest Branch, Mashhad, Iran
S
Masoudi
10031947532846003255
10031947532846003255
No
Department of Poutry Vaccines Research and Production, Razi Vaccine & Serum Research Institute, Karaj, Iran
N
Ghodsian
10031947532846003256
10031947532846003256
No
Department of Quality Control of Biological Products, Razi Vaccine & Serum Research Institute, Karaj, Iran
SR
Ebrahimi
10031947532846003257
10031947532846003257
No
Department of Poutry Vaccines Research and Production, Razi Vaccine & Serum Research Institute, Karaj, Iran
M
Abdoshah
10031947532846003258
10031947532846003258
No
Department of Quality Control of Biological Products, Razi Vaccine & Serum Research Institute, Karaj, Iran