en تخصصي Special پژوهشي Original article <div> <p><strong>Abstract</strong></p> <p><strong>Background and Aims:</strong> Multiple sclerosis (MS) is defined by the presence of inflammatory demyelinating plaques in the central nervous system (CNS). It has been indicated that human herpes virus 6 (HHV-6) may play role in pathogenesis of MS. The aim of this study was to determine the presence of HHV-6 DNA and anti HHV-6 IgG in MS patients and controls.</p> <p><strong>Materials and Methods:</strong> Blood samples from 59 patients with MS and 59 healthy controls were collected. DNA extraction and real-time PCR based on (Syber Green) were done in the peripheral blood mononuclear cells (PBMCs) and in plasma specimens. Anti-HHV-6 IgG was measured by ELISA technique. Demographic and clinical data were collected and entered in SPSS 16 and analyzed.</p> <p><strong>Results:</strong> HHV-6 DNA was found in the plasma sample of 1 (1.7%) of 59 MS patients. There was no HHV-6 DNA in PBMCs of the MS patients and controls and in plasma of control group. Anti- HHV-6 IgG antibody was present in 84.7% of MS patients and 82.1% of control group.</p> <p><strong>Conclusion: </strong>There was no significant relation based on presence of HHV-6 DNA and antibody between MS patients and controls in north of Iran.</p> </div> HHV-6, Multiple sclerosis, Real-Time PCR, ELISA 14 19 http://journal.isv.org.ir/browse.php?a_code=A-10-89-1&slc_lang=en&sid=1 Akramsadat Ahmadi akramsadat.ahmadi@yahoo.com 10031947532846003188 10031947532846003188 No Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran Mohammad Hasan Naeimi Dr_naeimii50@yahoo.com 10031947532846003189 10031947532846003189 No Department of Neurology, Sayyad Hospital, Golestan University of Medical Sciences, Gorgan, Iran Naeme Javid naeme_javid@yahoo.com 10031947532846003190 10031947532846003190 No Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran Abdolvahab Moradi abmoradi@yahoo.com 10031947532846003191 10031947532846003191 No Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran Mohammad Ali Vakili mavakili@yahoo.com 10031947532846003192 10031947532846003192 No Health Management and Social Development Research Center, Golestan University of Medical Sciences, Gorgan, Iran alijan Tabarraei alijant@yahoo.com 10031947532846003193 10031947532846003193 Yes Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran