Iranian Journal of Virology
مجله ویروس شناسی ایران
Iran J Virol
Medical Sciences
http://journal.isv.org.ir
1
admin
1735-5680
2588-5030
10.21859/isv
en
jalali
1394
5
1
gregorian
2015
8
1
9
4
online
1
fulltext
en
Rotavirus NSP4 protein as a viral biotoxin selectively promotes cytotoxicity
تخصصي
Special
پژوهشي
Original article
<div>
<p><strong>Abstract</strong></p>
<p><strong>Background and Aims:</strong> The number of new cancer cases with considerable mortality is increasing worldwide. Since the inability of current therapies in treatment of patients and prevention the progress of tumors with fewer side effects, implementation of new methods is needed. Gene therapy has widespread systemic cytotoxic effects against tumor cells. Rotavirus NSP4 has been shown to elicit extensive cytotoxic activities in transfected or infected cells. In this study, the biological cytotoxic effect of NSP4 rotavirus protein was investigation on TC-1 tumor cell integrity.</p>
<p><strong>Materials and Methods:</strong> NSP4 gene of rotavirus was cloned into the pCDH plasmid and then TC-1 tumor cells were transfected with plasmids. After reviewing the presence of this protein by SDS-PAGE and confirming NSP4 expression by western blotting using anti- NSP4 antibody, the cytotoxic effect of NSP4 expression in TC-1 tumor cells was measured by the MTT assay.</p>
<p><strong>Results:</strong> Significant differences were observed in the cell viability between the control groups and the group of cells that received NSP4 gene.</p>
<p><strong>Conclusion: </strong>Rotavirus NSP4 gene posses specific cell cytotoxicity and is potentially effective in tumor destruction.</p>
</div>
Rotavirus, NSP4, Enterotoxin, Cytotoxicity, Cancer Therapy
24
28
http://journal.isv.org.ir/browse.php?a_code=A-10-98-1&slc_lang=en&sid=1
Zohreh
Farahmand
zohre.farahmand@modares.ac.ir
10031947532846003146
10031947532846003146
No
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Hoorieh
Soleimanjahi
soleim_h@modares.ac.com
10031947532846003147
10031947532846003147
Yes
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Ehsan
Arefian
Aref58@yahoo.com
10031947532846003148
10031947532846003148
No
Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran
Zahra
Goodarzi
Goodarzi002@gmail.com
10031947532846003149
10031947532846003149
No
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Hadi
Razavinikoo
Hadi_razavi@yahoo.com
10031947532846003150
10031947532846003150
No
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Yasaman
Rezaie
Rezaie.yasaman@yahoo.com
10031947532846003151
10031947532846003151
No
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.