Iranian Journal of Virology
مجله ویروس شناسی ایران
Iran J Virol
Medical Sciences
http://journal.isv.org.ir
1
admin
1735-5680
2588-5030
10.21859/isv
en
jalali
1393
11
1
gregorian
2015
2
1
9
1
online
1
fulltext
en
Qualitative-based Multiplex PCR: Diagnosis of Papilloma virus Types 16 & 18 in Samples Taken from Patients with Malignant Lesions
تخصصي
Special
پژوهشي
Original article
<div>
<p><strong>Abstract</strong></p>
<p><strong>Background and Aims:</strong> The human papilloma virus was introduced as the major etiological agent in outbreak of cervical cancer in 1970. Since it is very difficult to recognize these viruses and their types using the serological tests and cell culture, molecular methods such as PCR are of great importance. Therefore, in this study, our goal was to use a multiple specific PCR assay on L1 and E6 genes of HPV for molecular detection of this virus and its common type’s detection in the society.</p>
<p><strong>Materials and Methods: </strong>After collecting the samples from malignant lesions of various patients, the viral DNA was extracted from paraffin blocks of 50 clinical samples and the PCR method was performed on the mentioned samples by specific primers for L1 and E6 genes together with β-globin (as internal control). The PCR product was analyzed on 2℅ agarose gel and the sensitivity of this test was examined.</p>
<p><strong>Results:</strong> from among 50 samples of the patients, 33 cases were HPV positive and 17 ones were negative. The sensitivity of this test was 20 copies from recombinant construct containing target genes for each reaction.</p>
<p><strong>Conclusion: </strong>this study confirmed that the designed PCR with specific primers on L1 and E6 genes of HPV proved to be an accurate method for detecting and determining the HPV types.</p>
</div>
Cancer of cervix, Human Papilloma Virus, PCR
13
20
http://journal.isv.org.ir/browse.php?a_code=A-10-43-30&slc_lang=en&sid=1
Mohammad Reza
Shafaati
10031947532846003221
10031947532846003221
Yes
Department of cellular & molecular biology, Faculty of Basic Sciences, Hamadan Branch, Islamic Azad University, Hamadan, Iran
Elnaz
Akhavan
10031947532846003222
10031947532846003222
No
Department of Microbiology, Faculty of Basic Sciences, Damaghan Branch, Islamic Azad University, Damghan, Semnan, Iran
Shahram
Yazdani
10031947532846003223
10031947532846003223
No
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Saeed
Mobini
10031947532846003224
10031947532846003224
No
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Mostafa
Ebadi
10031947532846003225
10031947532846003225
No
Department of Biology, Faculty of Sciences, Damaghan Branch, Islamic Azad University, Damghan, Semnan, Iran