en عمومى General پژوهشي Original article <div style="border-style: solid none border-top-color: windowtext border-top-width: 1pt border-bottom-color: windowtext border-bottom-width: 1pt padding: 1pt 0in"> <p class="MsoNormal" style="border: none padding: 0in"><b>Background and Aims:</b> Herpes simplex virus type 1 (HSV1) remains a potentially serious health problem world wide. All infected people, including asymptomatic ones, are potential sources for virus transmission. Virus envelope contains at least 13 glycoproteins, which glycoprotein D is the major target of immune responses. The aim of this study was development of a specific method that is a more rapid, sensitive and specific test compared to the virus neutralization test which is applied as gold standard test. <!--stripped--><!--stripped--></p> <p class="MsoNormal" style="border: none padding: 0in"><b>Methods: </b>In this study, the Western blot technique using crude HSV1 whole particle and baculovirus derived glycoprotein D of HSV1 as antigens was set. Human sera were analyzed by virus neutralization test and then serum samples with reciprocal virus neutralization antibody titers of 32, 64, and 128 were taken to be analyzed by Western blotting.<!--stripped--><!--stripped--></p> <p class="MsoNormal" style="border: none padding: 0in"><b>Results:</b> It was shown that there was a very good correlation between results obtained from virus neutralization antibody titers and those of Western blotting. Western blotting using recombinant glycoprotein D of HSV1 as an antigen showed positive results similar to the whole HSV1 antigen.<!--stripped--><!--stripped--></p> </div> <b><span style="font-size: 12pt font-family: 'Times New Roman', serif">Conclusion:</span></b><span style="font-size: 12pt font-family: 'Times New Roman', serif"> Our study showed the Western blotting using recombinant glycoprotein D can replace virus neutralization test in diagnosis of HSV1 infections.</span> Glycoprotein D, Herpes simplex virus type1, Recombinant gD, Virus neutralization test 13 17 http://journal.isv.org.ir/browse.php?a_code=A-10-1-3&slc_lang=en&sid=1 Z Meshkat 10031947532846002501 10031947532846002501 No Virology Department, Faculty of Medical Sciences, Tarbiat Modares University. Tehran, Iran H Soleimanjahi 10031947532846002502 10031947532846002502 Yes Virology Department, Faculty of Medical Sciences, Tarbiat Modares University. Tehran, Iran MH Roostaee 10031947532846002503 10031947532846002503 No Virology Department, Faculty of Medical Sciences, Tarbiat Modares University. Tehran, Iran T Bamdad 10031947532846002504 10031947532846002504 No Virology Department, Faculty of Medical Sciences, Tarbiat Modares University. Tehran, Iran M Meshkat 10031947532846002505 10031947532846002505 No Islamic Azad University, Mashhad Branch, Mashhad, Iran