en عمومى General پژوهشي Original article <div> <p><strong>Abstract</strong></p> <p><strong>Background and Aims:</strong> The burden of HIV infection and disease continues to be a major global public health concern all over the world. This study was conducted to measure IgG antibody against EBV viral capsid antigen (EBV-VCA IgG), Herpes Simplex Type1, and 2 to determine the seroprevalence of this infection in HIV-positive population.</p> <p><strong>Materials and Methods:</strong> A Cross-sectional study between March and August 2016 was conducted in keyvan virology laboratory enrolling 84 HIV-positive patients with different age and sexes. Enzyme-linked immunosorbent assay (ELISA) was used for determination of IgG antibody against Herpes Simplex Type1, Type 2 viruses, EBV and HIV viral load detected in plasma by Real time-PCR in obtained samples from HIV-positive patients.</p> <p><strong>Results:</strong> The overall seroprevalence was 66.7% for anti-HSV-1 IgG, 14.3% for anti-HSV-2 IgG, and 94% for anti-EBV IgG. There was no significant difference between the sex groups for HSV-1 and -2, EBV and HIV load and their IgG level. This study also showed a correlation between the age, and the antibody titers only for HSV-1 and -2 with P=0.030 and P= 0.024, respectively.</p> <p><strong>Conclusions: </strong>In our study, the seroprevalence of EBV and HSV-1 IgG were higher in HIV-positive patients. It can be derived that HSV-2 virus is not major coinfection in Iran, thus requires less attention than others, but annually monitoring needs for proper health care programs.</p> </div> Keywords: HIV, EBV, HSV-1, HSV-2, Sero-prevalence 29 34 http://journal.isv.org.ir/browse.php?a_code=A-10-107-1&slc_lang=en&sid=1 Atefeh Bahavar At.bahavar@gmail.com 10031947532846003152 10031947532846003152 No Department of Virology, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran Seyed Hamid Reza Monavari hrmonavari@yahoo.com 10031947532846003153 10031947532846003153 No Department of Virology, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran Hossein Keyvani keyvanlab@yahoo.com 10031947532846003154 10031947532846003154 No Department of Virology, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran Maryam Esghaei Maryam.esghaei@gmail.com 10031947532846003155 10031947532846003155 No Department of Virology, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran Saied Ghorbani vet.s.ghorbani@gmail.com 10031947532846003156 10031947532846003156 No Department of Virology, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran Angila Ataei-Pirkooh ataei.a@iums.ac.ir 10031947532846003157 10031947532846003157 Yes Department of Virology, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran