en تخصصي Special گزارش کوتاه Short communication <div> <p><strong>Abstract</strong></p> <p><strong>Background and Aims:</strong> Infectious bronchitis&nbsp;virus (IBV) is the causative agent of avian&nbsp;infectious bronchitis, which is characterized by respiratory, reproductive, and renal signs. IBV is a highly variable virus with a large number of genotypes.&nbsp;S1 gene sequencing has been used for molecular epidemiological studies and genotypic characterization of IBV. To better understand the molecular epidemiology of&nbsp;IBV in Iran, we sequenced the S1 gene of IBV field isolates, a total of 40 tracheal and kidney tissue specimens from different commercial broiler flocks in the East of Iran were collected from 2015.</p> <p>&nbsp;<strong>Materials and Methods: </strong>15 IBV-positive samples were selected from among the total and were further characterized by sequencing the spike glycoprotein gene.&nbsp;&nbsp; The isolates were confirmed by real-time polymerase chain reaction (PCR) and characterized by sequencing the spike glycoprotein gene.</p> <p><strong>Results:</strong> Three genotypes were detected. The percentage of Variant 2 (IS/1494 like), 793/B, and QX genotypes was 66.7%, 26.7%, and 6.6% respectively. We reported the QX as the first time and Variant2 was the dominant genotype in this area.</p> <p><strong>Conclusion: </strong>It is an updated and comprehensive study of genotyping of IBV and completes IBV puzzle in the East of Iran.</p> </div> Avian Infectious Bronchitis, Iran, Genotyping, Spike 31 35 http://journal.isv.org.ir/browse.php?a_code=A-10-110-2&slc_lang=en&sid=1 A Ghalyanchi-Langeroudi 10031947532846003204 10031947532846003204 Yes Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran V Karimi 10031947532846003205 10031947532846003205 No Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran A Jannat 10031947532846003206 10031947532846003206 No Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran M Hashemzadeh 10031947532846003207 10031947532846003207 No Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, Iran MH Fallah 10031947532846003208 10031947532846003208 No Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, Iran F Gholami 10031947532846003209 10031947532846003209 No Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran MT Zabihi 10031947532846003210 10031947532846003210 No Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran M Heidarzadeh 10031947532846003211 10031947532846003211 No Iranian Veterinary Organization, Zahedan , Iran