en عمومى General پژوهشي Original article <div style="border-style: solid none border-top-color: windowtext border-top-width: 1pt border-bottom-color: windowtext border-bottom-width: 1pt padding: 1pt 0in"> <p class="MsoNormal" style="direction: ltr unicode-bidi: embed border: none padding: 0in"><b>Background and Aims:</b> Adenovirus is one of the causative agents of viral conjunctivitis. Approximately an average of 40% of viral conjunctivitis is due to adenovirus infection. The rate of infection is usually the highest during the spring and summer months. In this study attempt was made to evaluate the incidence of conjunctivitis due to adenovirus infection in patients referred to one of the affiliated university hospital using the congenital virological methods.<!--stripped--><!--stripped--></p> <p class="MsoNormal" style="direction: ltr unicode-bidi: embed border: none padding: 0in"><b>Materials and Methods</b>: Samples were taken by a swab from patients with clinical conjunctivitis. Samples were processed and tested using the techniques of cell culture inoculation and polymerase chain reaction.<!--stripped--><!--stripped--></p> <p class="MsoNormal" style="direction: ltr unicode-bidi: embed border: none padding: 0in"><b>Results</b>: From the 100 samples taken 16% of then were positive by PCR Method. From these only 8% showed viral growth on cell culture. There was no difference of infection between the sex groups but most cases accrued in patients aged 17-27 years during the months of March to May.<!--stripped--><!--stripped--></p> <p class="MsoNormal" style="direction: ltr unicode-bidi: embed border: none padding: 0in"><b>Conclusion</b>: From the results of this study if was concluded that adenovirus plays a major role as a causative agent of conjunctivitis.<!--stripped--><!--stripped--></p> </div> Infectious bronchitis virus (IBV), Avian influenza virus (H9N2), Massachusetts, 793/B serotype, multiplex RT- PCR 7 11 http://journal.isv.org.ir/browse.php?a_code=A-10-1-10&slc_lang=en&sid=1 M Shamsi-Shahrabadi mshahrabadi@hotmail.com 10031947532846002539 10031947532846002539 Yes Department of Virology, Iran University of Medical Sciences, Tehran, Iran E Mousavi 10031947532846002540 10031947532846002540 No Department of Virology, Iran University of Medical Sciences, Tehran, Iran SHR Monavari 10031947532846002541 10031947532846002541 No Department of Virology and Antimicrobial Resistance Research Center, Iran University of Medical Sciences, Tehran, Iran A Ataei-Pirkooh 10031947532846002542 10031947532846002542 No Department of Virology, Iran University of Medical Sciences, Tehran, Iran P Bakhtiari 10031947532846002543 10031947532846002543 No Department of Ophthalmology, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran