en تخصصي Special پژوهشي Original article <div><strong>Background and Aims:</strong> The aim of this study was to produce and purify the Polyclonal antibody (pAb) against Matrix protein 2 (M2) with reasonable efficiency. Matrix protein 2 is one of the most conserved proteins of the influenza A virus which acts as ion channel. Polyclonal antibodyproduced against Matrix protein 2 is used in vaccine research, passive immunization and qualitative/quantitative analysis methods<span dir="RTL">.</span><br> <strong>Materials and Methods:</strong> Recombinant M2 protein was produced in E.coli. Purified protein with Freund&rsquo;s adjuvants (Complete and Incomplete) was injected into two New-Zealand white male rabbits. The polyclonal antisera of rabbits were evaluated by RID, immunoblotting and ELISA. The IgG was purified using DAEA-cellulose column chromatography. Finally, the quality and properties of purified IgG were evaluated using SDS-PAGE and ELISA<span dir="RTL">. </span><br> <strong>Results:</strong> The RID and immunoblotting results showed that the produced anti-M2 antibody was able to recognize M2 recombinant protein epitopes. The ELISA results confirmed anti-M2 pAb reached reasonable titers after three injections. IgG against M2 was purified with suitable concentration. The Purified polyclonal IgG-M2 was evaluated using ELISA and the results showed IgG-M2 reacted with the antigen up to 1:32000.<br> <strong>Conclusions: </strong>The data showed that recombinant M2 protein was able to stimulate immune response to produce antibody at satisfactory level.</div> ELISA, Influenza Virus, M2 protein, polyclonal antibody, RID. 31 38 http://journal.isv.org.ir/browse.php?a_code=A-10-214-1&slc_lang=en&sid=1 S Zamani s_zamani31@yahoo.com 10031947532846006761 10031947532846006761 No Department of Influenza and other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran/Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran F Fotouhi Chahouki fotouhi@pasteur.ac.ir 10031947532846006762 10031947532846006762 No Department of Influenza and other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran P Mehrbod mehrbode@yahoo.com 10031947532846006763 10031947532846006763 No Department of Influenza and other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran S Sadeghi Neshat bio_sa_n@yahoo.com 10031947532846006764 10031947532846006764 No Department of Influenza and other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran B Farahmand b_farahmand@pasteur.ac.ir 10031947532846006765 10031947532846006765 Yes Department of Influenza and other Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran