Iranian Journal of Virology
مجله ویروس شناسی ایران
Iran J Virol
Medical Sciences
http://journal.isv.org.ir
1
admin
1735-5680
2588-5030
10.21859/isv
en
jalali
1398
9
1
gregorian
2019
12
1
13
2
online
1
fulltext
en
Detection of Avian Encephalomyelitis Virus in Broiler Chickens in Iran Using RT-PCR and Histopathological Methods
تخصصي
Special
پژوهشي
Original article
<div><strong>Introduction: </strong>Avian encephalomyelitis is caused by a Tremovirus and primarily affects chickens. The virus can infect young chicks and cause nervous symptoms. Vaccines are used to control the disease in breeders. Recently, the occurrence of the disease is causing concern in the poultry industry.<br>
<strong>Materials and Methods:</strong> In the present study, we report a case of avian encephalomyelitis in one broiler farm in Kashan (center of Iran). The chickens showed nervous symptoms like depression, head trembling, ataxia, dull eyes, as well as drowsiness, lack of coordination, and unsteady gait in chickens. In this study, we detected avian encephalomyelitis with the molecular procedure like reverse transcriptase-polymerase chain reaction (RT-PCR). Also, the histopathological diagnosis was made. <br>
<strong>Results:</strong> The disease was confirmed based on the clinical, molecular and histopathological findings.<br>
<strong>Conclusions: </strong>Due to the increase in vaccine prices and the difficulties of vaccine providing in the breeders’ farms, the probability of disease has increased. Stringently monitoring of breeders farms before the beginning of egg production and using a suitable protocol with vaccination is recommended. </div>
avian encephalomyelitis, broiler, RT-PCR, histopathology, Iran
24
28
http://journal.isv.org.ir/browse.php?a_code=A-10-239-1&slc_lang=en&sid=1
Mohammadreza
Ghorani
mo_gh66@yahoo.com
10031947532846007070
10031947532846007070
No
Pathobiology Department, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Arash
Ghalyanchi Langeroudi
arashghalyanchi@gmail.com
10031947532846007071
10031947532846007071
Yes
Microbiology and Immunology Department, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Ali
Akramian
akramian_ali@yahoo.com
10031947532846007072
10031947532846007072
No
Veterinary Laboratory, Kashan, Iran
Hossein
Hosseini
hosseini.ho@gmail.com
10031947532846007073
10031947532846007073
No
Clinical Sciences Department, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran
Fatah
Rohani
fatahrohani@yahoo.com
10031947532846007074
10031947532846007074
No
Isfahan Veterinary Office, Isfahan, Iran