Iranian Journal of Virology
مجله ویروس شناسی ایران
Iran J Virol
Medical Sciences
http://journal.isv.org.ir
1
admin
1735-5680
2588-5030
10.21859/isv
en
jalali
1388
7
1
gregorian
2009
10
1
3
3
online
1
fulltext
en
Genetic Analysis of Hemagglutinin Protein of H9N2 Isolated from Live Bird Markets in Tehran Province in 2007-2008
عمومى
General
پژوهشي
Original article
Background and Aims: Influenza A virus subtype H9N2 have continued to circulate in domestic poultry farms in Asia since 1998. The virus circulates in live bird markets, missing link in epidemiology of avian influenza. Regarding previous studies on H9N2 viruses of Iran and having no data on this subject in Iranian live bird markets this study was conducted to analyze genetically hemagglutinin protein of H9N1 virus. Methods: A total of 500 tracheal and cloacal swabs from clinically healthy birds of Tehran's live bird markets were collected. Diagnostic RT-PCR was done on them using specific primers for subtype H9N2. Eight positive samples were selected for inoculation into 9-11 days SPF emberyonated eggs and the virus was grown and isolated. Amplification of the HA gene was carried out by PCR using two pairs of specific primers. PCR products were separated, purified and cloned. The products were sequenced and analyzed with M13 primers. They shared high amino acid homology with genes of other H9N2 viruses isolated previously in Asia and Iran. Results: H9N2 viruses isolated from live bird markets were highly similar to viruses isolated from industrial poultry being circulated as early as 2001. Conclusion: The results suggest that a common source of H9N2 viruses is circulating in Iran.
Avian influenza virus (H9N2), Live Bird Market, Hemagglutinin
7
15
http://journal.isv.org.ir/browse.php?a_code=A-10-1-17&slc_lang=en&sid=1
S
Ghadi
10031947532846002567
10031947532846002567
No
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
MH
Bozorgmehri-Fard
mhbfard@ut.ac.ir
10031947532846002568
10031947532846002568
Yes
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
V
Karimi
10031947532846002569
10031947532846002569
No
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
M
Tavassoti-Kheiri
10031947532846002570
10031947532846002570
No
Influenza Unit, Pasteur Institute of Iran, Tehran, Iran
MH
Rafiei
10031947532846002571
10031947532846002571
No
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran