en تخصصي Special پژوهشي Original article <div style="border-bottom:solid windowtext 1.0pt; border-top:solid windowtext 1.0pt; border-left:none; border-right:none; padding:1.0pt 0in 1.0pt 0in"><span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background and Aims: </span></b><span style="font-size:11.0pt">Enteroviruses are spread worldwide that cause many diseases, including gastrointestinal infections. These viruses are considered as an important pathogen in wastewater and therefore their presence in treated wastewater can cause disease transmission. We aimed to investigate the molecular prevalence and characterization of human enteroviruses in wastewater samples of a wastewater treatment in Tehran, Iran.</span></span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Materials and Methods: </span></b><span style="font-size:11.0pt">From November 2017 to April 2018, a total of 15 samples were collected by Grab sampling method from three chambers including the chamber adjacent to the aeration tank and the pre-chlorination effluent and the post-chlorination effluent. The samples were analysed for the presence of human enteroviruses by an integrated cell culture/polymerase chain reaction (ICC/PCR) technique. Then, the isolated enteroviruses were evaluated using sequencing analysis.</span></span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Results:</span></b><span style="font-size:11.0pt"> The results showed that the rate of infection with enteroviruses using culture method and RT-PCR technique in wastewater samples was 80% and 40%, respectively. Also, human enteroviruses in the samples were Coxsackievirus B5 (50%), poliovirus type 1 vaccine (33%) and Echovirus 11 (16%).</span></span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Conclusion: </span></b><span style="font-size:11.0pt">Human enteroviruses were detected in wastewater samples and the highest rate with coxsackievirus B5 (50%) and then with poliovirus type 1 vaccine (33%) and Echovirus 11 (16%)</span><span style="font-size:11.0pt">.</span></span></span></span></div> Wastewater, ICC-PCR, Human Enteroviruses 42 46 http://journal.isv.org.ir/browse.php?a_code=A-10-1-98&slc_lang=en&sid=1 Z Azarash 10031947532846007111 10031947532846007111 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. M Esghaei 10031947532846007112 10031947532846007112 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. M Farzadkia 10031947532846007113 10031947532846007113 No Department of Environmental Health Engineering, Faculty of Public Health, Iran University of Medical Sciences, Tehran, Iran. F Bokharaei-Salim 10031947532846007114 10031947532846007114 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. SJ Kiani 10031947532846007115 10031947532846007115 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. SH Monavari 10031947532846007116 10031947532846007116 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. G Mokhtary-Irani 10031947532846007117 10031947532846007117 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. MS Hosseini 10031947532846007118 10031947532846007118 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. A Ataei-Pirkooh 10031947532846007119 10031947532846007119 Yes Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.