en تخصصي Special پژوهشي Original article <div style="border-bottom:solid windowtext 1.0pt; border-top:solid windowtext 1.0pt; border-left:none; border-right:none; padding:1.0pt 0in 1.0pt 0in"><span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background and Aims: </span></b><span style="font-size:11.0pt">Human adenoviruses (HAdV) are worldwide distributed pathogens that cause a variety of illnesses, including gastrointestinal infections. These viruses are considered as important pathogens in wastewater, reclamation, and reused water. We aimed to investigate the molecular prevalence and characterization of HAdV in wastewater samples as well as air samples of aeration tanks of a wastewater treatment plant (WWTP) in Tehran, Iran.</span></span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Materials and Methods: </span></b><span style="font-size:11.0pt">From November 2017 to April 2018, a total of 14 wastewater and 9 air samples were collected and analyzed for the presence of HAdV by an integrated cell culture/polymerase chain reaction (ICC/PCR) technique. The samples were collected from the Ekbatan WWTP in the west of Tehran. To capture bio-aerosols, a liquid impingement biosampler was used. Typing of HAdV was performed by sequencing analysis.</span></span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Results:</span></b> <span style="font-size:11.0pt">Out of nine untreated</span> <span style="font-size:11.0pt">wastewater samples, one sample (11.1%) was positive for the presence of HAdV cytopathic effect (CPE), while two (40.4%) of five treated wastewater samples were positive for the HAdV CPE. The results of PCR assay also showed that 44.4 % (four out of 9 samples)</span> <span style="font-size:11.0pt">of the untreated wastewater and 60% (three out of 5 samples)</span> <span style="font-size:11.0pt">of the treated wastewater samples</span> <span style="font-size:11.0pt">were positive for HAdV genome. Regarding the air samples</span> <span style="font-size:11.0pt">of aeration tanks, four (44.4%) of nine samples were positive for the presence of the HAdV genome.</span> <span style="font-size:11.0pt">HAdV</span> <span style="font-size:11.0pt">in the wastewater samples were type B (40%), type C (40%), and type D (20%) and all four positive air samples for HAdV were type C</span><span style="font-size:11.0pt">.</span></span></span></span><br> <span style="font-size:12pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Conclusion: </span></b><span style="font-size:11.0pt">The human adenovirus detected in 50% of the wastewater and 44.44% of the air samples of the wastewater treatment plant of the Ekbatan, Tehran, Iran</span><span style="font-size:11.0pt">.</span></span></span></span></div> Human adenovirus, Waste Water, Molecular typing 38 43 http://journal.isv.org.ir/browse.php?a_code=A-10-1-102&slc_lang=en&sid=1 G Mokhtary-Irani 10031947532846007183 10031947532846007183 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. A Tavakoli 10031947532846007184 10031947532846007184 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran. University of Medical Sciences, Tehran, Iran. F Bokharaei-Salim 10031947532846007185 10031947532846007185 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. M Farzadkia 10031947532846007186 10031947532846007186 No Department of Environmental Health Engineering, Faculty of Public Health, Iran University of Medical Sciences, Tehran, Iran. A Tabibzadeh 10031947532846007187 10031947532846007187 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. SJ Kiani 10031947532846007188 10031947532846007188 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. M Esghaei 10031947532846007189 10031947532846007189 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. SH , Monavari 10031947532846007190 10031947532846007190 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. D Javanmard 10031947532846007191 10031947532846007191 No Infectious Diseases Research Center, Birjand University of Medical science, Birjand, Iran. Z Azarash 10031947532846007192 10031947532846007192 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. A Kachooei 10031947532846007193 10031947532846007193 No Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. A Ataei-Pirkooh 10031947532846007194 10031947532846007194 Yes Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.