Iranian Journal of Virology

en Expression Study of Subgenomic mRNAs of Avian Infectious Bronchitis, Variant 2, (GI-23), using RNA-Seq Analysis تخصصي Special پژوهشي Original article <div style="border-bottom:solid windowtext 1.0pt; border-top:solid windowtext 1.0pt; border-left:none; border-right:none; padding:1.0pt 0in 1.0pt 0in">Background and Aims: Infectious bronchitis virus (IBV), a member of the genus Gamma coronavirus under the Coronaviridae family, is one of the leading causes of persistent economic and financial burdens poultry industry of Iran. This family of viruses possesses a ~27 kb positive-sense RNA genome that produces a nested set of subgenomic mRNAs during replication. Materials and Methods: This study investigated tissue tropism of virulent IBV variant 2, GI-23, by comparing viral gene expression differences between kidney and trachea tissue samples of 20 intranasal infected SPF chickens. At 2 and 6 days post-infection, extracted genomic RNA was subjected to RNAseq, and high-throughput analyses studied the obtained data. Results: Among the genomic UTRs of IBV, the highest expression was obtained for the 3'UTR (7.15E+05) in the renal samples. Nucleoprotein (N) had the 2nd highest gene expression in both tissues (4.52E+05 in the trachea and 4.13E+05 in the kidney). The lowest expression (2.34E+03 in the trachea and 2.69E+02 in the kidney) belonged to the polymerase genes. 5'UTR expression was not detected in any of the tissue samples.Furthermore, six non-structural genes (NSP4, 7, 8, 9, 11, and 15) had no detectable expression in the kidney samples. NSP11 was shared in both tissues in this case, while NSP16 had the highest expression at 1.62E+05 in tracheal samples and NSP13 at 2.88E+5 in renal samples. Conclusion: We concluded that except for the 3’ UTR, a slightly higher expression was observed in tracheal samples.</div> Avian Infectious Bronchitis, RNA-Seq Analysis, Variant 2, IBV genome 36 41 http://journal.isv.org.ir/browse.php?a_code=A-10-1-106&slc_lang=en&sid=1 Zahra Ziafati Kafi 10031947532846006612 10031947532846006612 No Department of Microbiology and Immunology, University of Tehran, Faculty of Veterinary Medicine, Tehran, Iran. Aidin Molouki 10031947532846006613 10031947532846006613 No Department of Avian Diseases Research and Diagnostics, Razi Vaccine and Serum Research, Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. Hossein Hosseini 10031947532846006614 10031947532846006614 No Department of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran. Naser Sadri 10031947532846006615 10031947532846006615 No Department of Microbiology and Immunology, University of Tehran, Faculty of Veterinary Medicine, Tehran, Iran. Soroush Sarmadi 10031947532846006616 10031947532846006616 No Department of Microbiology and Immunology, University of Tehran, Faculty of Veterinary Medicine, Tehran, Iran. Mohammad Hossein Fallah Mehrabadi 10031947532846006617 10031947532846006617 No Department of Avian Diseases Research and Diagnostics, Razi Vaccine and Serum Research, Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. Ali Tolooe 10031947532846006618 10031947532846006618 No Department of Microbiology and Immunology, University of Tehran, Faculty of Veterinary Medicine, Tehran, Iran. Arash Ghalyanchilangeroudi 10031947532846006619 10031947532846006619 Yes Department of Microbiology and Immunology, University of Tehran, Faculty of Veterinary Medicine, Tehran, Iran.