en عمومى General پژوهشي Original article Background and Aims: Influenza A virus infects birds and some mammalian including human. H1 and H3 subtypes are circulated in both human and birds’ population. To determine the prevalence of the mentioned subtypes in birds, different captive bird species in Tehran zoo, and affiliated centers in Tehran were investigated for virus infection. Methods: In this study, Between November 2008 and February 2009, 76 cloacal swabs and serum samples were collected from 5 orders of Anseriformes, Galliformes, Columbiformes, Pelicaniformes and Phoenicopteriformes. Antibody surveillance was undertaken by haemagglutination inhibition assay and for detection of influenza virus genome RT-PCR technique was used. Results: In total, 57.7% and 88.75% of bird sera were seropositive against H1 and H3 viruses respectively. The highest GMT value and the greatest antibody titers were observed in Galliformes order particularly in chicken species. Influenza A genome was not detected in any of the samples by RT-PCR using M gene. Conclusion: Results of this study indicated that seropositive birds were infected during the last or possibly previous years with H1 and H3 virus strain. Hemagglutination Inhibition Test (HI), Real time polymerase chain reaction (RT-PCR), Captive birds, Tehran 7 16 http://journal.isv.org.ir/browse.php?a_code=A-10-1-31&slc_lang=en&sid=1 B Heydarchi 10031947532846002635 10031947532846002635 No Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, GC, Iran SM Hosseini 10031947532846002636 10031947532846002636 Yes Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, GC, Iran M Tavassoti-Kheiri 10031947532846002637 10031947532846002637 No Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran N Sheykhi 10031947532846002638 10031947532846002638 No Department of Clinical Sciences, Faculty of Specialized Veterinary Science, Science and Research Campus, Islamic Azad University, Tehran, Iran SF Mousavi 10031947532846002639 10031947532846002639 No Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, GC, Iran R Khaltabadi-Farahani 10031947532846002640 10031947532846002640 No Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, GC, Iran R Bashar 10031947532846002641 10031947532846002641 No Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran F Fotouhi 10031947532846002642 10031947532846002642 No Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran T Bolurieh 10031947532846002643 10031947532846002643 No Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran