Iranian Journal of Virology
مجله ویروس شناسی ایران
Iran J Virol
Medical Sciences
http://journal.isv.org.ir
1
admin
1735-5680
2588-5030
10.21859/isv
en
jalali
1390
8
1
gregorian
2011
11
1
5
4
online
1
fulltext
en
Development and Sequence Analysis of a Cold-Adapted Strain of Influenza A/New Caledonia/20/1999(H1N1) Virus
عمومى
General
پژوهشي
Original article
Background and Aims: Vaccination is the most effective method to prevent influenza infection. Among the available vaccines, cold-adapted live-virus vaccines are suitable approach that have been produced and evaluated for recent years in few countries. The goal of this project was to derivate a cold adapted variant of the influenza A/New Caledonia/20/1999(H1N1). Materials and Methods: Influenza A/New Caledonia/20/1999(H1N1) was adapted to grow at 25°C by gradually decreasing the incubation temperature through the sequential passages in embryonic eggs. The viral genome extracted from the starting seed and the last round of passage at 25°C was amplified by RT-PCR. The amplified cDNA fragments were subjected to sequencing determination bi-directionally. Sequence data were aligned to find mutated positions. Results: Sequence analysis showed totally six cases of point mutations that five of them resulted in amino acid substitutions and one of them was a silent mutation. These substitutions of one amino acid occurred in PB2, PA, NP proteins and two amino acid changes in HA protein sequence. Conclusion: The variant of cold adapted strain made here could be used as a master donor to generate attenuated reassortant influenza vaccine viruses.
Influenza Viruses Type A, cold adapted, vaccine, analysis
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10
http://journal.isv.org.ir/browse.php?a_code=A-10-1-55&slc_lang=en&sid=1
Z
Goodarzi
10031947532846003743
10031947532846003743
No
Applied Virology Research Center, Baqyatallah University of Medical Sciences, Tehran, Iran
F
Behzadian
10031947532846003744
10031947532846003744
No
Research Center for Sciences and Biotechnology, Tehran, Iran
E
Saberfar
10031947532846003745
10031947532846003745
Yes
Applied Virology Research Center, Baqyatallah University of Medical Sciences, Tehran, Iran