@article{ author = {Pourhossein, B and Soleimanjahi, H and Behzadian, F and Khansarinejad, B}, title = {Loop-Mediated Isothermal Amplification (LAMP) for the Rapid Diagnosis of Herpes Simplex Virus Type 1 (HSV-1)}, abstract ={Background and Aims: considering difficulties in usual laboratory methods in detection of viral infections, improved DNA-based diagnostic techniques are more reliable. Loop mediated isothermal amplification method (LAMP) is a nucleic acid amplification method that amplifies DNA using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. In this study, the LAMP was used for detection of Herpes simplex virus. Methods: The set of primers were designed, for detection of HSV-based on gG fragment. The genome of the virus was extracted from the culture supernatant of infected cells. LAMP technique was optimized in term of temperature, time and the ingredients used for test. For detection of HSV-1 infection, sensitivity and specificity of this technique were determined by various dilutions of virus and infected samples with HSV-2 that was taken from Day Hospital of Tehran. Results: The sensitivity and specificity of HSV-1 specific LAMP method, reached about 500 copies/tube and 99.9% respectively. Furthermore, both the agarose gel electrophoresis containing Ethidium Bromide (EtBr) and the turbidity assay directly detected HSV-1 virus LAMP products in reactions. Conclusion: In this study, the reliability of LAMP for detection of HSV- was approved therefore this rapid, accurate, and cost-effective detection and quantification method may perhaps be an investigative tool which can be valid for detection of target viral genome in clinical specimens in the absence of the necessary facilities for performing PCR.}, Keywords = {Loop mediated isothermal amplification method (LAMP), Herpes simplex virus type 1 (HSV-1), Sensitivity, Specificity}, volume = {5}, Number = {1}, pages = {1-5}, publisher = {Iranian Society for Virology}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.21859/isv.5.1.1}, url = {http://journal.isv.org.ir/article-1-59-en.html}, eprint = {http://journal.isv.org.ir/article-1-59-en.pdf}, journal = {Iranian Journal of Virology}, issn = {1735-5680}, eissn = {2588-5030}, year = {2011} } @article{ author = {Shahrabadi, MS and Ataei-Pirkooh, A}, title = {Accurance of Respiratory Syncytial Virus Infection in Children Referred to Kasra Hospital Diagnostic Laboratory during 2009-2011}, abstract ={Background and Aims: Respiratory syncytial virus (RSV) infection is common in infants and young children. In infants younger than one year old it may cause bronchiolitis and pneumonia which requires hospitalization. Accurate and rapid diagnosis of the disease will help proper treatment of the disease and prevents further complications. Methods: Specimen taken from respiratory tract of sick children were processed and fixed for immunofluorescence staining. Monoclonal antibody conjugate specific for RSV was used and the specimens were examined by a UV microscope. Results: A total of 132 samples examined, 40.1% were positive for RSV. The disease was mostly in children less than 6 months old. It occurred mainly during the winter months and there were no significant differences between the male and female children. Conclusion: RSV infects many infants and children annually during the winter. The virus probably infects considerable number of children across Iran. Proper diagnosis of the disease will help in treatment and control of the disease.}, Keywords = {Respiratory syncytial virus (RSV), Monoclonal antibody, Immunofluorescence Staining}, volume = {5}, Number = {1}, pages = {6-9}, publisher = {Iranian Society for Virology}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.21859/isv.5.1.6}, url = {http://journal.isv.org.ir/article-1-60-en.html}, eprint = {http://journal.isv.org.ir/article-1-60-en.pdf}, journal = {Iranian Journal of Virology}, issn = {1735-5680}, eissn = {2588-5030}, year = {2011} } @article{ author = {Khoshkhatti, N and Habibi-Koohi, M and Mosahebi, G}, title = {Characterization of Celery Mosaic Virus from Celery in Tehran Province}, abstract ={Background and Aims: Celery mosaic virus (CeMV) is one of the causal agents of viral diseases in celery (Apium graveolens). CeMV is a member of the Potyvirus genus in Potyviridae family. The virus is naturally transmitted by aphids in a non-persistent manner. During growing season 2006-2007 viral disease symptoms were observed in celery fields grown in Tehran Province (Bage daneshkade, Mohamadshahr, Varamin, Asgarabad, Hesarak and Savojbolagh) and suspected CeMV infected plants were sampled. Methods: Serological assays with specific antisera against CeMV and other potyviruses revealed that 5.42% of the samples were infected with CeMV in Tehran province. Biologically purified CeMV isolate was mechanically inoculated on test plants for host range studies. Results: The virus caused chlorotic local lesions on Chenopodium quinoa and C. amaranticolor. It also produced mosaic, vein clearing and leaf deformations symptoms on Apium graveolens. Molecular mass of CeMV coat protein was estimated about 38.36 kDa using SDS-PAGE and was approved by western blotting using CeMV specific polyclonal antibody. A DNA fragment of about 650bp was amplified by RT-PCR using CeMV infected crude RNA and universal primer pair for potyviruses coat protein genome. Conclusion: This is the first study on this virus in Iran (Tehran Province).}, Keywords = {Celery Mosaic Virus, Serological assays, Province}, volume = {5}, Number = {1}, pages = {10-14}, publisher = {Iranian Society for Virology}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.21859/isv.5.1.10}, url = {http://journal.isv.org.ir/article-1-61-en.html}, eprint = {http://journal.isv.org.ir/article-1-61-en.pdf}, journal = {Iranian Journal of Virology}, issn = {1735-5680}, eissn = {2588-5030}, year = {2011} } @article{ author = {Soleimani, S and Lotfi, M and Taghavian, M and Shahkarami, MK}, title = {Evaluation of Adventitious Agents in MMR & Oral Poliomyelitis Vaccines}, abstract ={Background and Aims: Adventitious agents, especially viral agents are among the most important concerns in viral vaccines. Viral contamination of biological products may arise from the original source of the reagents such as, serum, trypsin, animal or human derived media components or cell culture and working seed or cross contamination of vaccine during production. In this study, adventitious agents were evaluated in the MMR and oral polio vaccines. Methods: For detection of adventitious agents, two techniques were used. The suspensions that were harvested after inoculation on MRC-5 were neutralized with specific antisera and inoculated into Vero, HeLa and MRC-5 cells (in vitro tests). The suspensions that harvested after inoculation of CHEF primary cell culture were neutralized with specific antisera and inoculated in to Vero, MRC-5, CHEF, chorioallantoic membrane and yolk sac of SPF embryonated eggs (in vivo tests). Results: As indications of viral contamination, CPE in cell culture and the viability of egg embryo were observed and haemadsorption and haemagglutination test were performed. Finally, data were analyzed by exact binomial test (version 2.8.1). There was not any CPE in cell culture, the inoculated embryos were viable and there was not any haemadsorption & haemagglutination in all of the samples. Conclusion: The major focus of this Study will be to ensure that vaccines are devoid of adventitious agents. There were not any signs of viral agents in the samples and the preparations could be used for vaccine production.}, Keywords = {Adventitious Agents, Measles, Mumps, Rubella, Poliomyelitis}, volume = {5}, Number = {1}, pages = {15-22}, publisher = {Iranian Society for Virology}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.21859/isv.5.1.15}, url = {http://journal.isv.org.ir/article-1-62-en.html}, eprint = {http://journal.isv.org.ir/article-1-62-en.pdf}, journal = {Iranian Journal of Virology}, issn = {1735-5680}, eissn = {2588-5030}, year = {2011} } @article{ author = {Ataei-Pirkooh, A and Shahrabadi, MS and Haghi-Ashtiani, MT}, title = {Incidence of Coinfection between Rotavirus and Some Enteropathogenic Agents in Children Referred to Children Medical Center Hospital, Tehran, 2009}, abstract ={Background and Aims: One of the major causes of acute diarrhea in children during the cold season in Iran is infection by rotavirus. Children infected with rotavirus often require hospitalization. Several studies on human and animals have shown that enterotoxigenic Escherichia.coli and rotavirus are the most common coinfection causing diarrhea. There are other reports indicating occurrence of rotavirus coinfection with some other enteric agents such as Salmonella, Giardia, and Shigella flexeneri. In this study the rate of rotavirus infection and its coinfection with some other enteropathogenic agents in children which could influence the severity of the disease was investigated. Methods: Approximately 100 stool samples were collected from children with acute gastroenteritis. The specimens were cultured for bacteria isolation. They were also clarified and tested for rotavirus using the techniques of latex agglutination and negative staining electron microscopy. Results: Using negative staining, rotavirus particles were observed in 43 of the 100 stool specimen. The highest prevalence was observed in 6-12 months old children consisting 39.5% of the total specimens. Patients with mixed infection particularly rotavirus and E.coli had the highest incidence of severe vomiting and dehydration. Conclusion: Coinfection of children with rotavirus and other enteric agents can occur frequently. This coinfection has a synergistic effect which increases the severity of the clinical manifestation.}, Keywords = {Rotavirus, Escherichia.coli, enteropathogenic agents, coinfection}, volume = {5}, Number = {1}, pages = {23-27}, publisher = {Iranian Society for Virology}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.21859/isv.5.1.23}, url = {http://journal.isv.org.ir/article-1-63-en.html}, eprint = {http://journal.isv.org.ir/article-1-63-en.pdf}, journal = {Iranian Journal of Virology}, issn = {1735-5680}, eissn = {2588-5030}, year = {2011} } @article{ author = {Rangbar-Kermani, R and Sharifi, Z and Mahmoodian-Shooshtari, M and Mousavi, K}, title = {Quantitative Analysis of CMV-DNA Load in Renal Transplant Recipients Using Real-Time PCR}, abstract ={No abstract}, Keywords = {CMV, Renal transplant}, volume = {5}, Number = {1}, pages = {28-30}, publisher = {Iranian Society for Virology}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, doi = {10.21859/isv.5.1.28}, url = {http://journal.isv.org.ir/article-1-64-en.html}, eprint = {http://journal.isv.org.ir/article-1-64-en.pdf}, journal = {Iranian Journal of Virology}, issn = {1735-5680}, eissn = {2588-5030}, year = {2011} }