ORIGINAL_ARTICLE Evaluation of Humoral and Cell-mediated Immunity of Two Capripoxvirus Vaccine Strains against Lumpy Skin Disease Virus Background and Aims: Prevention of Lumpy skin disease (LSD) in cattle is heavily dependent on vaccination. Since the genetic structure of LSD virus has the close relatedness with other Capri-pox virus (CaPV). Therefore, the use of vaccine strains of CaPV derived from sheep and goat would be useful to protect cattle against LSD. Materials and Methods: The susceptible calves of dairy farms were vaccinated with two available LSD vaccines. These vaccines were live attenuated sheep pox and goat pox vaccine strains. To evaluate vaccine-induced immune responses, whole blood and serum samples were collected up to 5 weeks post vaccination from both vaccinated and control groups. Results: The findings showed that, lymphocyte proliferation index in response to recall antigen in goat pox vaccine was higher than sheep pox vaccine in all time-point of experiments, and this difference was significant in weeks 1 and 5 post vaccination (p<0.05). Although the levels of antibody production in both vaccinated groups was almost the similar, and there was no statistically significant difference, but in goat pox vaccine slightly higher than sheep pox vaccine. Also, the interferon gamma and IL-4 production in goat pox vaccine were higher than sheep pox vaccine in all-time point and statistically significant at week 3 post vaccination (p< 0.05). Conclusions: From this study we found that live attenuated goat pox vaccine induced high level of lymphocyte proliferation and interferon gamma and IL-4, so it considered good vaccine to control of Lumpy skin disease. http://journal.isv.org.ir/article-1-306-en.pdf 2017-10-14 1 11 10.21859/isv.10.4.1 LSD Capri Pox Virus Vaccine Reza Norian norian.reza@yahoo.com 1 Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran AUTHOR Nahideh Afzal Ahangaran n.a.ahangran@gmail.com 2 Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran AUTHOR Hamid reza Vashovi norian.reza@gmail.com 3 Department of Animal Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, Iran. AUTHOR Abbas Azadmehr azadmehr2010@gmail.com 4 Department of Immunology, Babol University of Medical Sciences, Mazandaran, Iran. AUTHOR
ORIGINAL_ARTICLE Hepatitis E Virus Seroprevalence and Viremia in Hemodialysis and HIV Infected Patients in Iran Background and Aims: Hepatitis E virus (HEV) infection is usually a self-limited viral disease that causes acute hepatitis and may progress to chronic hepatitis in immunosuppressed individuals. It seems that hemodialysis patients and HIV infected people are more exposed to HEV infection. The aim of this study was to evaluate the extent of HEV infection in hemodialysis and HIV infected patients in Iran using serological molecular methods. Materials and Methods: Serum and plasma samples were collected from 149 patients undergoing hemodialysis and also 102 proved HIV infected patients. Theses sera were used for detection of HEV total antibodies with Enzyme immunoassay and HEV RNA by Real Time PCR. Demographic and clinical data were obtained and analyzed by SPSS version 16. Results: HEV antibody for hemodialysis patients and HIV infected individuals were (4%) and (33.3%) respectively. No viremia was observed in both HIV and hemodialysis serum samples. There was no association between demographic and clinical data and HEV antibody positive people. Conclusions: This study showed some different results in comparison with other studies in Iran. These conflicting results showed differences between HEV infection in hemodialysis and HIV-infected patients in Iran. http://journal.isv.org.ir/article-1-273-en.pdf 2017-10-14 12 17 10.21859/isv.10.4.12 Hepatitis E HIV Hemodialysis serology viremia Nazila Hajiahmadi Nazila.hajiahmadi@yahoo.com 1 Msc of virology, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran AUTHOR Abdolvahab Moradi Amoradi@yahoo.com 2 PhD of virology, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran AUTHOR Mohammad Ali Vakili vakil@i goums.ac.ir 3 PhD of Statistic, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran AUTHOR Naeme Javid naeme_javid@yahoo.com 4 Msc of microbiology, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran AUTHOR Mishar Kelishadi kelishady@yahoo.com 5 Msc of virology, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran AUTHOR Masoud Bazouri M.bazourii@gmail.com 6 BS of microbiology, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran AUTHOR Alijan Tabarraei tabarraei@goums.ac.ir 7 PhD of virology, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran AUTHOR
ORIGINAL_ARTICLE Full-length Characterization of S1 Gene of Iranian QX Avian Infectious Bronchitis Virus Isolates, 2015 Background and Aims: Avian infectious bronchitis (IB) has prevalent in the most chicken farms during recent years, in spite of the IB vaccination program which has been widely performed in Iran. To better understand the molecular epidemiology of IBV in Iran, the full length sequences of S1 gene of Iranian QX IBVs were determined and phylogenetic analysis was done using some sequences of IBV. Materials and Methods: To better understand the molecular epidemiology of IBV in Iran, the full length sequences of S1 gene of Iranian QX IBVs were determined and phylogenetic analysis was done using some sequences of IBV. Results: Iranian QX IBVs were located in LX4 (Cluster 2). The nucleotides homologies were 99.52% - 100% between the isolates. Phylogenetic analysis revealed that all the IBV isolates were very similar and probably had a common origin. The hyperactive variable regions of S1 were determined. Conclusions: The results from this study and other published results in the GenBank database showed that the isolates circulating in Iran in recent years were mainly LX4 (Cluster 2) genotype, which is the predominant genotype circulating in Iran in recent years (After first report of QX IBV in Iran, 2011). This finding provides important information on IBV evolution in Iran. http://journal.isv.org.ir/article-1-272-en.pdf 2017-10-14 18 25 10.21859/isv.10.4.18 Avian Infectious Bronchitis QX Iran Phylogenetic analysis Spike Homayoon Davam 1 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AUTHOR Arash Ghalyanchi langeroudi 2 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AUTHOR Masoud Hashemzadeh 3 Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, Iran AUTHOR Vahid Karimi 4 Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AUTHOR Taha Zabihi 5 Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AUTHOR Waleed Seger 6 Department of Pathology and poultry diseases, Faculty of Veterinary Medicine, University of Basra, Basra, Iraq AUTHOR Hamideh Najafi 7 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AUTHOR
ORIGINAL_ARTICLE Nucleotide and Amino Acid Changes in HN, F and SH genes of an Iranian Mumps Virus; RS-12, Following Attenuation to Vaccine Strain Background and Aims: Wild-type RS-12 strain of mumps virus has been isolated from an Iranian patient and has been attenuated after several serial passages. This study was designed to determine nucleotide and amino acid substitutions in the HN, F and SH genes during attenuation of the wild-type virus. Materials and Methods: Required viral samples prepared at Razi Vaccine and Serum Institute. Viral RNA was extracted, targeted segments were amplified and sequenced and finally were analyzed using DNAMAN software. Results: No difference in nucleotide and deduced amino acid sequence of the F and SH genes was detected following attenuation of wild-type as compared to the vaccine strain, but four nucleotide changes in HN gene had occurred which had resulted in two nucleotide differences. Conclusions: Considering the HN nucleotide sequences and the deduced amino acid sequences, RS-12 wild-type and vaccine strains were distinguishable. Moreover, unique differences between RS-12 and some other vaccine strains existed. During serial passages of RS-12 strain for seed lot system preparation, no change in HN, F and SH genes occurred which -at least in some extents- proved the genetic stability of the attenuated RS-12 surface proteins. Potential attenuating mutations in other genes (N, P, M, L and even non-coding regions in 3′ and 5′ ends) should be investigated and confirmed using advanced methods http://journal.isv.org.ir/article-1-283-en.pdf 2017-10-14 26 33 10.21859/isv.10.4.26 RS-12 mumps virus vaccine Mohammad Kazem Shahkarami k.shahkarami@rvsri.ac.ir 1 Razi Vaccine and Serum Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran. Tel. +98 2634570038 AUTHOR Mohammad Taqavian m.taqavian@rvsri.ac.ir 2 Razi Vaccine and Serum Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran. Tel. +98 2634570038 AUTHOR Behnam Alirezaie b.alirezaie@rvsri.ac.ir 3 Razi Vaccine and Serum Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran. Tel. +98 2634570038 AUTHOR
ORIGINAL_ARTICLE Evaluation of Quality of Two Commercial Oil-Emulsion Newcastle Disease Vaccines In-vivo and In-vitro Background and Aims: Newcastle disease (ND) is a highly contagious disease in poultry with economic losses in the world. Vaccination is one of the most important ways for prevention and control of NDV, but there are reports of ND outbreaks in vaccinated chickens. Poor quality of the vaccines is one of the reasons of vaccine failure. In this study the quality of two commercial oil-emulsion Newcastle disease virus (OE-NDV) vaccines was evaluated in in-vitro and in-vivo. Materials and Methods: For in-vitro study,  the amount of total protein and quantity of retrieved hemagglutination activity (HA) of the vaccines were recovered and measured, then, the amount of recovered total protein and retrieved HA were compared with the serologic responses induced in chickens by each vaccine(in-vivo study). Results: The results showed that the extracted total protein , recovered HA titer, and Mean HI titers to vaccine A are higher than vaccine B.The results also indicated that there is a good correlation between retrieved HA activity, extracted total protein and antibody response to vaccine A, but about vaccine B, there is no good correlation between total protein and retrieved HA activity and antibody response. Conclusions: Quality of Vaccine B is lower than vaccine A, likely because of many reasons particularly improper storage of the vaccine, inappropriate harvesting of egg allantoic fluid and inactivation of NDV http://journal.isv.org.ir/article-1-254-en.pdf 2017-10-14 34 38 10.21859/isv.10.4.34 Hemagglutination activity Total protein vaccine potency Hemagglutination inhibition Zolfaghar Rajabi rajabi@tabrizu.ac.ir 1 Poultry diseases division, Clinical Sciences Department, Faculty of veterinary medicine, university of Tabriz, Tabriz, Iran AUTHOR Amirhossein Aslnajjari a.aslnajjari@gmail.com 2 Resident in poultry diseases at faculty of veterinary medicine, University of Shiraz, Shiraz, Iran AUTHOR
OTHERS_CITABLE Solving the Mystery of Dengue in Iran; Are We Close to an Answer? Dengue fever is a mosquito-borne infection which is endemic in Southeast Asia. Although it has not been distributed in Iran, locating near other countries where the Dengue has been revealed is a threat for Iran because different connection between countries may increase the rate of infection. http://journal.isv.org.ir/article-1-277-en.pdf 2017-10-14 39 40 10.21859/isv.10.4.39 Dengue Viruses Flaviviridae Aedes Iran Mostafa Salehi-Vaziri mostafavaziri1985@gmail.com 1 assistant professor AUTHOR Mehdi Fazlalipour mfp.virology@gmail.com 2 assistant professor AUTHOR Vahid Baniasadi baniasadi@pasteur.ac.ir 3 assistant professor AUTHOR