Iranian Society for Virology
Iranian Journal of Virology
1735-5680
2588-5030
6
4
2012
11
1
The Absence of Hepatitis C Virus Infection Among Patients with Hepatitis B virus in Mashhad, Iran
1
6
EN
S
Rostami
Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
N
MT
Shakeri
Department of Biostatistics, Public Health School, Mashhad University of Medical Sciences, Mashhad, Iran
N
M
Ghayour-Mobarhan
Biochemistry of Nutritional Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
N
H
Nomani
Microbiology and Virology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
N
S
Sepahi
Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
N
S
Gerayli
Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
N
B
Foghanian
Microbiology and Virology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
N
M
Sadat-Nabavinia
Department of Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
N
M
Ahadi
Department of Internal Medicine, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
N
Z
Meshkat
Women’s Health Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Y
10.21859/isv.6.4.1
Background and Aims: Many studies have provided evidence for the role of hepatitis B and C viruses in the development of liver cancer. Although the routine treatment is available for both conditions, no definite guideline is available to treat patients dually infected with HBV and HCV. This study was performed to determine the frequency of HBV/HCV-coinfection in Mashhad, North-East of Iran. Materials and Methods: In our previous study, 3198 participants were chosen for study of HBV infection from March 2010 to November 2011 in Mashhad, Iran. ELISA method was used to determine the existence of anti-HCV antibody among HBV infected cases. Results: Of 34 HBsAg positive participants that included equal number of men and women (17 subjects of each gender) with the mean age of 49.9 years, none were positive for anti-HCV antibody. Conclusion: According to the current study, it could be concluded that the prevalence of HBV/HCV coinfection is low in Mashhad. However, since occult HBV infection may go unnoticed by conventional HBsAg testing, high sensitive molecular techniques are required to investigate the presence of dual infection.
Co-infection, hepatitis B virus, hepatitis C virus, Mashhad, Iran
http://journal.isv.org.ir/article-1-101-en.html
http://journal.isv.org.ir/article-1-101-en.pdf
Iranian Society for Virology
Iranian Journal of Virology
1735-5680
2588-5030
6
4
2012
11
1
Molecular Identification of Fowl Adenovirus Associated with Inclusion Body Hepatitis in Iran
7
12
EN
H
Hosseini
Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran
Y
R
Morshed
Agriculture and Veterinary group, Iranian institute for encyclopedia research, Ministry of science, research and technology, Tehran, Iran
N
10.21859/isv.6.4.7
Background and Aims: Inclusion body hepatitis (IBH) is one of the most important reemerging diseases in many countries with intensive poultry industries. In Iran, the etiological agent of IBH (fowl adenovirus) has not been yet confirmed. The aim of this study was the molecular detection and identification of fowl adenovirus involving IBH in chicken flocks in Iran. Materials and Methods: Polymerase chain reaction (PCR) and sequence analysis of L1 hexon gene was utilized to detect and to determinate the genotypes of Fowl adenovirus (FAdV) in broiler breeder flocks. Histopathological sections were prepared and examined. Results: FAdVs were detected in livers. Based on sequencing analysis of the hexon gene, they were genetically related to FAdV-11, a member of the fowl adenovirus D species, with 98% homology to Korean strain in 2011. Histological examination revealed necrotizing hepatitis with basophilic intranuclear inclusion bodies in the hepatocytes. Conclusion: This study provided evidence for the role of fowl adenoviruses as agents, causing this clinical disease in Iran and indicated the importance of accurate diagnosis and prevention in the meat-type flocks.
Inclusion Body Hepatitis (IBH), Fowl Adenovirus Serotype 11, Breeder, Iran
http://journal.isv.org.ir/article-1-102-en.html
http://journal.isv.org.ir/article-1-102-en.pdf
Iranian Society for Virology
Iranian Journal of Virology
1735-5680
2588-5030
6
4
2012
11
1
Over Expression of Influenza Virus M2 Protein in Prokaryotic System
13
19
EN
MA
Alavi-Esfahani
Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran
N
F
Fotouhi-Chahooki
Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran
Y
M
Saleh
Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran
N
R
Tavakoli
Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran
N
B
Farahmand
Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran
N
A
Ghaemi
Department of Medical Virology and Immunology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
N
M
Tavassoti-Kheiri
Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran
N
10.21859/isv.6.4.13
Background and Aims: Influenza A virus of Orthomyxoviridae family is able to create pandemic influenza. Vaccination is the most effective way to prevent influenza virus infection. Matrix protein 2 (M2) is a homotetramer ion channel with 97 amino acids length and highly conserved among influenza viruses and is considered for development of a universal influenza vaccine. Materials and Methods: We present here cloning and expression of influenza A virus M2 protein as a fusion with 6-His tag in Escherichia coli BL21 strain. The gene was amplified by PCR and ligated into the prokaryotic expression vector pET28a. The expression of M2 protein was induced by IPTG and confirmed by SDS-PAGE and western blotting. The desired protein was purified with affinity chromatography on a Ni-TED resin column and has to be evaluated in animal models for further studies. Results: The results of sequencing showed that M2 gene was cloned in pET28a properly in frame to histidine tag and the product was confirmed by xpreimmune reaction of monoclonal anti-M2 antibody to recombinant M2 in western blotting. Conclusion: This study might provide a basis for production of a universal and broad-spectrum human influenza vaccine.
M2 Protein, Influenza Virus, Vaccine, pET28
http://journal.isv.org.ir/article-1-103-en.html
http://journal.isv.org.ir/article-1-103-en.pdf
Iranian Society for Virology
Iranian Journal of Virology
1735-5680
2588-5030
6
4
2012
11
1
Evaluation of Cell Mediated Immunity following Rubella Vaccination Using Lymphocyte Proliferation
20
26
EN
E
Jafari
Microbiology;Virology Department, Kerman University of Medical Sciences, Kerman, Iran
N
A
Mohammadi
Human viral vaccines Department, Razi Vaccine and Serum Research Institute, Karaj, Iran
N
SAM
Arabzadeh
Microbiology;Virology Department, Kerman University of Medical Sciences, Kerman, Iran
N
F
Esna-Ashari
Human viral vaccines Department, Razi Vaccine and Serum Research Institute, Karaj, Iran
Y
ZA
Sadigh
Human viral vaccines Department, Razi Vaccine and Serum Research Institute, Karaj, Iran
N
GhR
Shokri
Human viral vaccines Department, Razi Vaccine and Serum Research Institute, Karaj, Iran
N
R
Shahbazi
Human viral vaccines Department, Razi Vaccine and Serum Research Institute, Karaj, Iran
N
A
Foroughi
Human viral vaccines Department, Razi Vaccine and Serum Research Institute, Karaj, Iran
N
10.21859/isv.6.4.20
Background and Aims: Rubella is predominantly a childhood disease that is endemic throughout the world and when rubella outbreaks occur, they are accompanied by birth defects following congenital rubella syndrome. Immunity to rubella virus as a teratogenic agent has an important role for prevention of these serious congenital defects. Lymphocyte proliferation assay is a way for investigation of human cell-immunity and its ability against rubella infection. Materials and Methods: The blood samples were obtained in sodium heparin tubes. Ficoll was added to separate lymphocytes. The cells were cultured with RPMI 1640 medium with 15% calf serum in microplates and incubating at 37°C in 3-5% CO2. Mitogens including Phytohemagglutinin and rubella hemagglutinin antigen (derived Takahashi strain) were added, separately. Then a fluorescent nucleotide was added. On day 10th-11th the wells stained and observed. Results: Lymphocytes stimulated with the mitogens were observed directly with an inverted microscope. Their aggregation and growth were detected after two days. Also lymph proliferation was shown using labeled nucleotide comprising a new fluorophore, by fluorescent microscopy. Response to full particle of attenuated virus was better than antigens derived from different parts of the virus. Conclusion: Comparison of the data with previous studies on proliferation of specific lymphocytes in response to rubella vaccination confirms our results. Thus cell-immunity to rubella infection was activated timely, in individuals who were vaccinated against rubella virus approximately 10 years before or exposed to it, but the intensity of responses to different antigens varied in each subject.
Rubella, Vaccination, Cell-Mediated Immunity, Fluorescence Microscopy
http://journal.isv.org.ir/article-1-104-en.html
http://journal.isv.org.ir/article-1-104-en.pdf
Iranian Society for Virology
Iranian Journal of Virology
1735-5680
2588-5030
6
4
2012
11
1
Induction of Nucleic Acid Damage in Viral Genomes Using Riboflavin in Combination with UV Light
27
32
EN
H
Mirshafiee
Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
N
SM
Hosseini
Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
Y
Z
Sharifi
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
N
H
Latifi
Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran
N
F
Yari
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
N
H
Nikbakht
Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran
N
A
Elikaei
Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
N
10.21859/isv.6.4.27
Background and Aims: Despite the screening of blood donors, blood transfusion represents an ideal port of entry for blood-borne infection. Blood-borne pathogen transmission has been a concern since the earliest days of transfusion. The blood product of platelet (PLT) concentrates is still faced with the risk of bacterial and viral contaminations. Pathogen inactivation technologies offer a proactive approach and the potential to further improve blood safety. Here we study the pathogen inactivating capacity of riboflavin with UV light treatment in platelet concentrates contaminated with enveloped and non-enveloped viruses. Materials and Methods: The inactivation effects of riboflavin in combination with UV light was examined on Herpes simplex virus (HSV), Vesicular stomatitis virus (VSV) and Polio virus classified as enveloped and non-enveloped DNA and RNA viruses, respectively. After spiking viruses in PLT concentrate, treatment was undertaken with riboflavin (50 µM) and exposed to different doses of UV light. Residual viral infectivity was titrated using 50%Tissue Culture Infective Dose (TCID50). Results: Combination of Riboflavin and UV light treatment reduced the titer of HSV, VSV and Poliovirus with different doses of UV light. Log reduction of HSV with UV doses of 0.82, 1.63, 2.44 and 3.25 J/cm2 was 0.5, 1.3, 3.5 and 3.8, respectively. Log reduction of VSV for 0.82, 1.63, 2.44 and 3.25 J/cm2was 2.8, 3.8, 4.6 and 5.6, respectively. Also, log reduction of poliovirus for 0.82, 1.63, 2.44 and 3.25 J/cm2was 1.7, 2.5, 2.7 and 3.1 respectively. Conclusion: The final dose of UV (3.25 J/cm2) resulted in a larger amount of viral inactivation for enveloped and non-enveloped viruses, suspended in PLT. This method offers a potential to be used for prevention of the majority of PLT transfusion-associated viral pathogens.
Riboflavin, UV Light, Platelet Concentrate, Virus Inactivation
http://journal.isv.org.ir/article-1-105-en.html
http://journal.isv.org.ir/article-1-105-en.pdf
Iranian Society for Virology
Iranian Journal of Virology
1735-5680
2588-5030
6
4
2012
11
1
Molecular Surveillance of Avian Influenza in Live Bird Market of Qom City in Iran
33
34
EN
K
Majidzadeh-A
Tasnim Biotechnology Research Center (TBRC), Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
N
M
Soleimanidor
Tasnim Biotechnology Research Center (TBRC), Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
N
A
Morovvati
Tasnim Biotechnology Research Center (TBRC), Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
N
V
Karimi
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
N
A
Ghalyanchi-Langeroudi
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Y
10.21859/isv.6.4.33
No abstract this article:Molecular Surveillance of Avian Influenza in Live Bird Market of Qom City in Iran
Avian Influenza, Live Bird Market, Qom
http://journal.isv.org.ir/article-1-106-en.html
http://journal.isv.org.ir/article-1-106-en.pdf