1 1735-5680 Iranian Society for Virology 306 Special Evaluation of Humoral and Cell-mediated Immunity of Two Capripoxvirus Vaccine Strains against Lumpy Skin Disease Virus Norian Reza b Afzal Ahangaran Nahideh c Vashovi Hamid reza d Azadmehr Abbas e b Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran c Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran d Department of Animal Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, Iran. e Department of Immunology, Babol University of Medical Sciences, Mazandaran, Iran. 1 12 2016 10 4 1 11 18 06 2017 14 10 2017 Background and Aims: Prevention of Lumpy skin disease (LSD) in cattle is heavily dependent on vaccination. Since the genetic structure of LSD virus has the close relatedness with other Capri-pox virus (CaPV). Therefore, the use of vaccine strains of CaPV derived from sheep and goat would be useful to protect cattle against LSD. Materials and Methods: The susceptible calves of dairy farms were vaccinated with two available LSD vaccines. These vaccines were live attenuated sheep pox and goat pox vaccine strains. To evaluate vaccine-induced immune responses, whole blood and serum samples were collected up to 5 weeks post vaccination from both vaccinated and control groups. Results: The findings showed that, lymphocyte proliferation index in response to recall antigen in goat pox vaccine was higher than sheep pox vaccine in all time-point of experiments, and this difference was significant in weeks 1 and 5 post vaccination (p<0.05). Although the levels of antibody production in both vaccinated groups was almost the similar, and there was no statistically significant difference, but in goat pox vaccine slightly higher than sheep pox vaccine. Also, the interferon gamma and IL-4 production in goat pox vaccine were higher than sheep pox vaccine in all-time point and statistically significant at week 3 post vaccination (p< 0.05). Conclusions: From this study we found that live attenuated goat pox vaccine induced high level of lymphocyte proliferation and interferon gamma and IL-4, so it considered good vaccine to control of Lumpy skin disease.
273 General Hepatitis E Virus Seroprevalence and Viremia in Hemodialysis and HIV Infected Patients in Iran Hajiahmadi Nazila f Moradi Abdolvahab g Vakili Mohammad Ali h Javid Naeme i Kelishadi Mishar j Bazouri Masoud k Tabarraei Alijan l f Msc of virology, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran g PhD of virology, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran h PhD of Statistic, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran i Msc of microbiology, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran j Msc of virology, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran k BS of microbiology, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran l PhD of virology, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran 1 12 2016 10 4 12 17 26 04 2017 14 10 2017 Background and Aims: Hepatitis E virus (HEV) infection is usually a self-limited viral disease that causes acute hepatitis and may progress to chronic hepatitis in immunosuppressed individuals. It seems that hemodialysis patients and HIV infected people are more exposed to HEV infection. The aim of this study was to evaluate the extent of HEV infection in hemodialysis and HIV infected patients in Iran using serological molecular methods. Materials and Methods: Serum and plasma samples were collected from 149 patients undergoing hemodialysis and also 102 proved HIV infected patients. Theses sera were used for detection of HEV total antibodies with Enzyme immunoassay and HEV RNA by Real Time PCR. Demographic and clinical data were obtained and analyzed by SPSS version 16. Results: HEV antibody for hemodialysis patients and HIV infected individuals were (4%) and (33.3%) respectively. No viremia was observed in both HIV and hemodialysis serum samples. There was no association between demographic and clinical data and HEV antibody positive people. Conclusions: This study showed some different results in comparison with other studies in Iran. These conflicting results showed differences between HEV infection in hemodialysis and HIV-infected patients in Iran. 272 General Full-length Characterization of S1 Gene of Iranian QX Avian Infectious Bronchitis Virus Isolates, 2015 Davam Homayoon m Ghalyanchi langeroudi Arash n Hashemzadeh Masoud o Karimi Vahid p Zabihi Taha Seger Waleed Najafi Hamideh m Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran n Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran o Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, Iran p Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Avian Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Pathology and poultry diseases, Faculty of Veterinary Medicine, University of Basra, Basra, Iraq Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran 1 12 2016 10 4 18 25 24 04 2017 14 10 2017 Background and Aims: Avian infectious bronchitis (IB) has prevalent in the most chicken farms during recent years, in spite of the IB vaccination program which has been widely performed in Iran. To better understand the molecular epidemiology of IBV in Iran, the full length sequences of S1 gene of Iranian QX IBVs were determined and phylogenetic analysis was done using some sequences of IBV. Materials and Methods: To better understand the molecular epidemiology of IBV in Iran, the full length sequences of S1 gene of Iranian QX IBVs were determined and phylogenetic analysis was done using some sequences of IBV. Results: Iranian QX IBVs were located in LX4 (Cluster 2). The nucleotides homologies were 99.52% - 100% between the isolates. Phylogenetic analysis revealed that all the IBV isolates were very similar and probably had a common origin. The hyperactive variable regions of S1 were determined. Conclusions: The results from this study and other published results in the GenBank database showed that the isolates circulating in Iran in recent years were mainly LX4 (Cluster 2) genotype, which is the predominant genotype circulating in Iran in recent years (After first report of QX IBV in Iran, 2011). This finding provides important information on IBV evolution in Iran. 283 Special Nucleotide and Amino Acid Changes in HN, F and SH genes of an Iranian Mumps Virus; RS-12, Following Attenuation to Vaccine Strain Shahkarami Mohammad Kazem Taqavian Mohammad Alirezaie Behnam Razi Vaccine and Serum Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran. Tel. +98 2634570038 Razi Vaccine and Serum Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran. Tel. +98 2634570038 Razi Vaccine and Serum Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran. Tel. +98 2634570038 1 12 2016 10 4 26 33 07 06 2017 14 10 2017 Background and Aims: Wild-type RS-12 strain of mumps virus has been isolated from an Iranian patient and has been attenuated after several serial passages. This study was designed to determine nucleotide and amino acid substitutions in the HN, F and SH genes during attenuation of the wild-type virus. Materials and Methods: Required viral samples prepared at Razi Vaccine and Serum Institute. Viral RNA was extracted, targeted segments were amplified and sequenced and finally were analyzed using DNAMAN software. Results: No difference in nucleotide and deduced amino acid sequence of the F and SH genes was detected following attenuation of wild-type as compared to the vaccine strain, but four nucleotide changes in HN gene had occurred which had resulted in two nucleotide differences. Conclusions: Considering the HN nucleotide sequences and the deduced amino acid sequences, RS-12 wild-type and vaccine strains were distinguishable. Moreover, unique differences between RS-12 and some other vaccine strains existed. During serial passages of RS-12 strain for seed lot system preparation, no change in HN, F and SH genes occurred which -at least in some extents- proved the genetic stability of the attenuated RS-12 surface proteins. Potential attenuating mutations in other genes (N, P, M, L and even non-coding regions in 3′ and 5′ ends) should be investigated and confirmed using advanced methods 254 Special Evaluation of Quality of Two Commercial Oil-Emulsion Newcastle Disease Vaccines In-vivo and In-vitro Rajabi Zolfaghar Aslnajjari Amirhossein Poultry diseases division, Clinical Sciences Department, Faculty of veterinary medicine, university of Tabriz, Tabriz, Iran Resident in poultry diseases at faculty of veterinary medicine, University of Shiraz, Shiraz, Iran 1 12 2016 10 4 34 38 06 12 2016 14 10 2017 Background and Aims: Newcastle disease (ND) is a highly contagious disease in poultry with economic losses in the world. Vaccination is one of the most important ways for prevention and control of NDV, but there are reports of ND outbreaks in vaccinated chickens. Poor quality of the vaccines is one of the reasons of vaccine failure. In this study the quality of two commercial oil-emulsion Newcastle disease virus (OE-NDV) vaccines was evaluated in in-vitro and in-vivo. Materials and Methods: For in-vitro study,  the amount of total protein and quantity of retrieved hemagglutination activity (HA) of the vaccines were recovered and measured, then, the amount of recovered total protein and retrieved HA were compared with the serologic responses induced in chickens by each vaccine(in-vivo study). Results: The results showed that the extracted total protein , recovered HA titer, and Mean HI titers to vaccine A are higher than vaccine B.The results also indicated that there is a good correlation between retrieved HA activity, extracted total protein and antibody response to vaccine A, but about vaccine B, there is no good correlation between total protein and retrieved HA activity and antibody response. Conclusions: Quality of Vaccine B is lower than vaccine A, likely because of many reasons particularly improper storage of the vaccine, inappropriate harvesting of egg allantoic fluid and inactivation of NDV 277 General Solving the Mystery of Dengue in Iran; Are We Close to an Answer? Salehi-Vaziri Mostafa Fazlalipour Mehdi Baniasadi Vahid assistant professor assistant professor assistant professor 1 12 2016 10 4 39 40 20 05 2017 14 10 2017 Dengue fever is a mosquito-borne infection which is endemic in Southeast Asia. Although it has not been distributed in Iran, locating near other countries where the Dengue has been revealed is a threat for Iran because different connection between countries may increase the rate of infection.