[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Contact ::
:: Volume 6, Issue 3 (8-2012) ::
Iran J Virol 2012, 6(3): 19-23 Back to browse issues page
Development of a Multiplex Polymerase Chain Reaction for Differential Diagnosis of Canary Pox Virus
MM Ebrahimi *, S Shahsavandi, S Masoudi, N Ghodsian, A Hashemi, MH Hablalvarid, AR Hatami
Razi vaccine & serum Research Institute, Karaj, Iran
Abstract:   (3980 Views)
Background and Aims: A multiplex transcription-polymerase chain reaction (m-PCR) was developed for direct detection and discrimination between canarypox virus (CPV) and other avian poxvirus (APV). Materials and Methods: Three compatible primer sets were designed for m-PCR amplification of different loci fpv126, fpv140, and fpv167 located at highly conserved APV genes. Results: Results showed that m-PCR products of the expected sizes were obtained for all of the primer sets when they were tested either alone or in combination with an artificial mixture of positive controls. Based on the better amplification of fpv167 than other loci, the locus primer set was used to examine tissue samples from canaries clinically diagnosed as AVP-infected. Conclusion: All canary samples were positive for CPV by the m-PCR and virus isolation. The results of the present study indicate the m-PCR assay holds potential to be versatile, rapid, and sensitive for detection of CPV and differentiation of the virus from the other APVs.
Keywords: Canary pox virus, Fragment-Length Polymorphism, Diagnosis
Full-Text [PDF 391 kb]   (1789 Downloads)    
Type of Study: Original article | Subject: General
Received: 2014/11/7 | Accepted: 2014/11/7 | Published: 2014/11/7
Add your comments about this article
Your username or Email:


XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ebrahimi M, Shahsavandi S, Masoudi S, Ghodsian N, Hashemi A, Hablalvarid M et al . Development of a Multiplex Polymerase Chain Reaction for Differential Diagnosis of Canary Pox Virus. Iran J Virol 2012; 6 (3) :19-23
URL: http://journal.isv.org.ir/article-1-98-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 6, Issue 3 (8-2012) Back to browse issues page
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4540