|
Development a New Human Skin Continuous Cell Line Sensitive to Mumps Virus
|
N Mahmudi-Gharoeie   , A Mohammadi * , B Saffar , F Esna-Ashari , A Foroghi , B Alirezaee , R Ghorbani , ZA Sedigh |
| Human Viral Vaccine Department, Razi Vaccine and Research Institute, Alborz Karaj, Iran |
|
|
Abstract: (4895 Views) |
| Background and Aims: During the last years a new Foreskin cell line strain (RFSC) has been isolated, characterized and stored in a representative cell bank stock in liquid nitrogen as a part of HCB (Human Cell Bank) development program in Razi institute. Materials and Methods: The cell population was serially passaged for mass production purpose based on WHO schedule .The controls on pool samples were carried out upon our sampling method and international recommendations. Results: The data concerning parameters as well as the result of compulsory controls on Kario logy oncogenicity, cross contaminations, species specificity, no oncogenicity, and mycoplasma free of a human cell line that contained heteroploid chromosomal characterizations are performed. Conclusion: Kariological examination found an obvious discrepancy in the frequency of aberrations due to high passage level that produced a heteroploid continuous cell line. Virus susceptibility shows RFSC-1 more sensitive to RS#12 Mumps virus strain. This cell line is completely sterile and has no any cross contamination to other pathogen and can be used for production and control of Mumps vaccine. |
|
| Keywords: Primary culture, Cell Characterization, Human Cell Bank, RFSC-1 |
|
|
Full-Text [PDF 474 kb]
(1462 Downloads)
|
Type of Study: Original article |
Subject:
General
Received: 2015/08/21 | Accepted: 2015/08/21 | Published: 2015/08/21
|
|
|
|
|
|
