Abstract

Background and Aims: To identify and isolation of viruses a susceptible cell is need which can be primary or line. The use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. IRKHBK cell line was originated from primary cells of bovine kidney by continuous passage. The aim of this study was to introduce the IRKHBK cell line for isolation, propagation and diagnosis of animal viruses.

Material and Methods: Samples of the infected animals, such as the lymph nodes, tonsils, lungs and blood were used for virus isolation. Primary BK cell and IRKHBK cell line cultures were inoculated with the prepared samples simultaneously under the same conditions. Identification of the isolated viruses was carried out with virus neutralization test using standard antibodies.

Results: Bovine Herpes Virus-1, Parainfluenza-3, Bovine viral diarrhea, and Peste des Petits Ruminants Virus were easily isolated and propagated in IRKHBK cells. The viral titer was higher in IRKHBK cell line compared to the primary BK cell. The IRKHBK cell line also showed considerable susceptibility in serological tests.  

Conclusion: The results show that IRKHBK cell line can be used for virus isolation, detection and propagation. This cell line can be used for animal and human viruses’ research studies and also other pathogens that are able to grow in the cell culture.