Background: Dengue fever is a mosquito-borne disease which is not known to be endemic in Iran.

Case Report: In October 2015, a 32-year-old Iranian woman was admitted with acute unexplained high-grade fever, headache, pain, rash, diarrhea, leukopenia and elevated liver enzymes after returning from India. Serological and molecular analysis for Dengue virus (DENV) infection revealed positive IgM, negative IgG and positive reverse transcriptase-PCR (RT-PCR)

Results: PCR product was sequenced and the phylogenetic analysis showed a DENV 2, genotype 4 strain with high similarity to other isolates reported from India.

Conclusion: Considering that DENV is one of the most common infections among travelers, an integrated surveillance system is strictly recommended in dengue non endemic countries.