[صفحه اصلی ]   [Archive]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
بخش‌های اصلی
آرشیو مجله و مقالات::
برای نویسندگان::
برای داوران::
ثبت نام و اشتراک::
تماس با ما::
تسهیلات پایگاه::
بایگانی مقالات زیر چاپ::
::
جستجو در پایگاه

جستجوی پیشرفته
..
:: دوره 11، شماره 1 - ( 12-1395 ) ::
جلد 11 شماره 1 صفحات 31-23 برگشت به فهرست نسخه ها
Development of RT-PCR Using External and Internal Positive Controls Based on 5' Untranslated Region (UTR) for Molecular Detection of Avian Infectious Bronchitis Virus
چکیده:   (4054 مشاهده)
Background and Aims: Infectious bronchitis virus (IBV) belongs to the group of gamma coronaviruses along with other avian coronaviruses. The disease caused by IBV can appear similar to infectious laryngotracheitis, avian influenza, and velogenic Newcastle disease, which are high priority diseases. The clinical signs can be accompanied by mortalities in broiler chickens and reduced eggshell and albumin quality in layer hens, leading to economic loss for the poultry industry. Rapid detection of IBV is useful for implementation of control measures, research purposes, and understanding the epidemiology and evolution of IBVs. The aim of the present study was the rapid identification of IB with the molecular method, which targets the 5’ untranslated region (UTR) gene of IBV that is less variable than the other genes, with homologies greater than 90% among IBV strains.
Materials and Methods: The primers designed to amplify a conserved fragment of the gene. Analytical sensitivity and specificity of the assay were determined.
Results: The results of specificity exhibited the specific amplification of the designed primers for IBV. Sensitivity was 10 pg/μl of the pTZ57R/T-5' UTR. This is the first report of RT-PCR method coupled with construction of comparative internal positive control (IPC) according to 5´UTR gene for accurate detection of IBV. 100 fg/μl of the IPC amplified in the presence of the limit of detection (10 pg/μl) of 5' UTR gene was determined as the optimal concentration of IPC plasmid for RT-PCR of clinical specimens.
Conclusions: The RT-PCR assay presented provides a time saving, sensitive, and reliable method for detection of IBV.
متن کامل [PDF 1138 kb]   (2410 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: تخصصي
دریافت: 1396/10/23 | پذیرش: 1396/10/23 | انتشار: 1396/10/23
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA


XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Madhi A, Ghalyanchi-langeroudi A, Hosseini S, Soleimani M, Mohseni A. Development of RT-PCR Using External and Internal Positive Controls Based on 5' Untranslated Region (UTR) for Molecular Detection of Avian Infectious Bronchitis Virus. Iran J Virol 2017; 11 (1) :23-31
URL: http://journal.isv.org.ir/article-1-320-fa.html

Development of RT-PCR Using External and Internal Positive Controls Based on 5' Untranslated Region (UTR) for Molecular Detection of Avian Infectious Bronchitis Virus. مجله ویروس شناسی ایران. 1395; 11 (1) :23-31

URL: http://journal.isv.org.ir/article-1-320-fa.html



بازنشر اطلاعات
Creative Commons License این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.
دوره 11، شماره 1 - ( 12-1395 ) برگشت به فهرست نسخه ها
Persian site map - English site map - Created in 0.04 seconds with 37 queries by YEKTAWEB 4645