Background and Aims: Inclusion body hepatitis (IBH) associated with FAdV-I ( fowl adenovirus) occurs worldwide in the poultry industry. It is an acute disease mainly affecting young chickens between 3 and 7 weeks of age. The objective of this study was to detect and establish the molecular sequences of the hexon gene from the avian adenovirus strains of group I (FAdV-I) from birds to characterize the serotype of the virus affecting commercial flocks in Iran.
Materials and Methods: Samples were obtained from 2-week old broiler flocks of Ross from Golestan province of Iran on March 2018. DNA was extracted by Sina Pure DNA extraction kit. The 590 bp region of the hexon gene was amplified using a pair of specific primers. The PCR products were sequenced in the forward and reversed direction.
Results: The nucleotide sequences of the hexon gene showed the existence of FAdV-8b from species E among broilers of Golestan province.