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:: Volume 12, Issue 2 (12-2018) ::
Iran J Virol 2018, 12(2): 19-24 Back to browse issues page
Novel RFLP Method for Detection of Four Avian Infectious Bronchitis Virus Genotypes (Massachusetts, 793/B, Variant2, and QX)
Vahid Karimi , Arash Ghalyanchilangeroudi * , Fatemeh Gholami , Masoud Hasehmzadeh , AmirHossein Mohseni , Hossein Hosseini , Naser Sadri , Amir Modiri Hamdan
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Abstract:   (2076 Views)
Background and Aims: Infectious bronchitis (IB) is one of the significant economically critical poultry diseases distributed worldwide, including Iran. Different IB virus (IBV) genotypes are circulating in different geographical regions. Typing of IBV strains is useful for implementation of control measures and for understanding the epidemiology and evolution of IBV. The S1 gene sequencing is used for IBV genotyping. Massachusetts, 793/B, QX, and IS-14194 IBV genotypes currently exist in Iranian poultry farms. This study aims to design an RFLP-based method to do genotyping and confirm of mentioned IBV genotypes.
Materials and Methods: After amplification of parts of the S1 gene (RT-PCR), the PCR products treated with four enzymes (SfoI: Massachusetts, BtsCI: 793/B, MspI: IS-1494, and NruI: QX) and finally visualized on agarose gel electrophoresis.
Results: Results showed 100 percent of the specificity of the newly designed method (in comparison with sequencing).
Conclusions: This method can be used for primary confirmation and fast screening of Massachusetts, 793/B, QX, and IS-14194 IBV genotypes and even in local laboratories without the need for sequencing.
Keywords: Avian Infectious Bronchitis, Iran, RFLP, Genotyping
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Type of Study: Original article | Subject: Special
Received: 2019/07/27 | Accepted: 2019/11/27 | Published: 2019/11/27
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Karimi V, Ghalyanchilangeroudi A, Gholami F, Hasehmzadeh M, Mohseni A, Hosseini H, et al . Novel RFLP Method for Detection of Four Avian Infectious Bronchitis Virus Genotypes (Massachusetts, 793/B, Variant2, and QX). Iran J Virol 2018; 12 (2) :19-24
URL: http://journal.isv.org.ir/article-1-356-en.html

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