Background and Aims: Rotaviruses are the main cause of gastroenteritis in the young of human and a variety of animals in the world. Rotavirus G6 and G10 are the most prevalent genotypes in cattle worldwide, but are also found in human with possible origination from animals.

Materials and Methods: In this work, we have detected the G6 and G10 genotypes of bovine rotaviruses isolated from diarrheic calves in Tehran province by RT-PCR. The amplified VP7 genes were cloned, sequenced and compared to the sequences deposited in the GenBank database from the world.

Results: The phylogenetic analysis showed that the Iranian G6 and G10 genotypes are most identical to the genotypes from Ireland (>98.6%  amino acid homology) and Canada (>97.3% amino acid homology), respectively.

Conclusion: This high similarity between the Iranian sequences and those from the far countries may suggest that the virus can be spread in a wide geographic region possibly by animal transportation.

Background and Aims: Infectious bronchitis (IB) is an economically important disease of chickens. The existence of very large number of IBV serotypes and variants which insufficiently induce cross protection against each other is the major problem to control the disease.
Materials and Methods: This study was performed to characterize S1, N and 3'UTR region of the genome of H-120 and H-52 vaccine strains of Razi Vaccine Institute. The S1, N and 3'UTR region were sequenced and compared with standard strain in gene bank.
Results: Based on nucleotide identity, the S1, N and 3'UTR region of the genome of Iranian IBV vaccine strain showed 100% similarity to the commonly standard IBV strains. To better characterization of these strains analysis of other genes involved in virulence and pathogenesis of the virus and performing protective tests against field strains are recommended.
Conclusions: The results revealed that H-52 and H-120 strains of Razi institute were identical to the standard of strains in the Gene Bank.