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Background and Aims: Adenovector gene transfer induces inflammatory response that finally leads to vector removal from delivered site. The effect of hydrodynamic pre-injection on Adenovector mediated liver inflammation remained elusive. Materials and Methods: Different mice groups were pre-treated by hydrodynamic saline, dexamethasone or nothing prior to Adeno-Luciferase administration. Their samples were collected in 18 hours later and serum IL-1β level as a marker of inflammation was quantified. Also, liver histological assessment was performed to score the inflammatory grade. Results and Conclusion: In spite of dexamethasone group, both hydrodynamic/Ad-Luc and Ad-Luc receiving groups induced the expression of IL-1β cytokine (p<0.05). The pathological views also indicated more inflammation and necrosis inside the different zones of liver tissue in these two adenovector injected groups. These results emphasized that Adenovector served as a suitable inflammatory model and hydrodynamic injection enhances this kind of inflammation.