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Showing 2 results for Fowl Pox Vaccine
Bahman Khalesi, Mohammad Majid Ebrahimi, Naser Ghodsian, Amir Kaffashi, Mohammadkazem Shahkarami, Mir Saeed Ebrahimzadeh,
Volume 13, Issue 1 (6-2019)
Abstract
Background and Aims: Fowl pox vaccine is produced in the Razi Institute for almost half a century and has a favorable and productive yield in poultry flocks and has provided complete satisfaction to the poultry breeder. In terms of comparing the efficacy of this vaccine with imported vaccines, the following research was conducted.
Materials and Methods: In this study, based on the latest protocols of European Pharmacopeia and OIE, 100 SPF chicks were divided into five groups: the first group was given 20 SPF chickens at the age of 8 to 10 weeks of domestic vaccine (Razi Institute: Live attenuated (inoculation in the wing with the needle of the twin branches); also in the second to fourth group: to 60 SPF chicks (in three groups of 20) at the age of 8 to 10 weeks; imported vaccines (I, C and H) were injected. Finally, in the fifth group, 20 chicks were considered as controls and did not receive vaccine. Response of the immune system was observed 7 to 10 days after vaccination by observing nodules at the injection site (Takes reaction). At 21 days (three weeks), all four groups vaccinated with acute pathogenesis of fowl pox strain were challenged. Chickens were observed daily for 21 days after vaccination and the results of vaccination immunization were evaluated and analyzed by statistical analysis.
Results: The results of the experiments indicated that after vaccination, 100% of the vaccinated chickens were positive by takes responses and after being challenged in four groups vaccinated in the Razi, I, C vaccines 100% and vaccine H, 95% of the immune responses were observed lesions in the Crown of the birds, and in the control group, there were symptoms of cartilage like in 100% of the birds.
Conclusions: In general, according to the OIE standard, the above experiments showed that fowl pox vaccine Razi Institute induces high immunity and has efficacy similar to imported vaccines.
Mahdi Khalili Gheidariy, Bahman Khalesi, Mostapha Ghaderi, Morteza Taghizadeh, Mohammad Kazem Shahkarami, Hossein Karimi Razakani,
Volume 14, Issue 2 (12-2020)
Abstract
Abstract Background and Aims: For more than half a century, the production of fowl pox vaccine at Razi Vaccine and Serum Research Institute, has been carried out by injection method in the chick chorioallantoic membrane (CAM) and the vaccine has a favorable and effective in poultry flocks and has provided a complete satisfaction to the poultry flocks owner. Fowl pox vaccine is also manufactured using chicken embryo cell (CEF) culture in other countries. The aim of this project is to develop fowl pox vaccine based on CEF which is of vital importance and a requirement for Razi institute. Materials and Methods: In this study, chicken fibroblastic cells were used as primary cell culture in Hanks or DMEM media supplemented with fetal bovine serum 10% (FBS). First the cells were cultured and the cell count was determined. Subsequently, the virus was added to the cells. The virus that used to prepare the vaccine was initially grew up in the fibroblast cells and had a titer of 106.3 TCID50/ml. To determine the viral load, two methods plaque-forming unit (PFU) and TCID50 were used, safety and efficacy tests were performed on 10 chickens, and the potency test on 20 chickens, and vaccinated chickens were challenged with wild fowl pox virus strain. Results: The results of the tests showed that the vaccinated chickens had an adequate and sufficient resistance to the acute form of fowl pox virus. Conclusion: In total, according to the OIE standard, the above experiments showed that cell culture-based fowl pox vaccine can generate good immunity response and have high efficacy.
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