%0 Journal Article %A Mahmudi-Gharoeie, N %A Mohammadi, A %A Saffar, B %A Esna-Ashari, F %A Foroghi, A %A Alirezaee, B %A Ghorbani, R %A Sedigh, ZA %T Development a New Human Skin Continuous Cell Line Sensitive to Mumps Virus %J Iranian Journal of Virology %V 7 %N 4 %U http://journal.isv.org.ir/article-1-143-en.html %R 10.21859/isv.7.4.7 %D 2013 %K Primary culture, Cell Characterization, Human Cell Bank, RFSC-1, %X Background and Aims: During the last years a new Foreskin cell line strain (RFSC) has been isolated, characterized and stored in a representative cell bank stock in liquid nitrogen as a part of HCB (Human Cell Bank) development program in Razi institute. Materials and Methods: The cell population was serially passaged for mass production purpose based on WHO schedule .The controls on pool samples were carried out upon our sampling method and international recommendations. Results: The data concerning parameters as well as the result of compulsory controls on Kario logy oncogenicity, cross contaminations, species specificity, no oncogenicity, and mycoplasma free of a human cell line that contained heteroploid chromosomal characterizations are performed. Conclusion: Kariological examination found an obvious discrepancy in the frequency of aberrations due to high passage level that produced a heteroploid continuous cell line. Virus susceptibility shows RFSC-1 more sensitive to RS#12 Mumps virus strain. This cell line is completely sterile and has no any cross contamination to other pathogen and can be used for production and control of Mumps vaccine. %> http://journal.isv.org.ir/article-1-143-en.pdf %P 7-13 %& 7 %! %9 Original article %L A-10-43-1 %+ Human Viral Vaccine Department, Razi Vaccine and Research Institute, Alborz Karaj, Iran %G eng %@ 1735-5680 %[ 2013