en تخصصي Special <div> <p><strong>Abstract: </strong>Peganum harmala L. (Zygophylaceae) has been used in a variety of practical applications in medical science. Our objective in the current study was to determine the effects of the seed extract on Herpes simplex virus type 1 (HSV-1) replication in Vero cells. Different concentrations (100, 500, 571, 667, 800 and 1000 &micro;g/ml) of the extract were examined. Experiments were carried out using Vero cells. P.harmala seed extract was found to be non-toxic to Vero cells up to a concentration of 667 &micro;g/ml. The antiviral activity of non-toxic concentration against HSV-1 was tested. The replication of HSV-1 was inhibited, indicating that the P.harmala L. extract contains an anti-HSV-1 substance.&nbsp;</p> </div> Peganum harmala L. ,Vero cells ,TCID50 ,Cytotoxicity 11 16 http://journal.isv.org.ir/browse.php?a_code=A-10-43-24&slc_lang=en&sid=1 S.J Kiani 10031947532846002387 10031947532846002387 Yes Department of Virology, Iran University of Medical Sciences, Tehran, IRAN M Shamsi Shahrabadi Mshahrabadi@hotmail.com 10031947532846002388 10031947532846002388 No Department of Virology, Iran University of Medical Sciences, Tehran, IRAN A Ataei 10031947532846002389 10031947532846002389 No Department of Virology, Iran University of Medical Sciences, Tehran, IRAN N Sajjadi 10031947532846002390 10031947532846002390 No Department of Virology, Iran University of Medical Sciences, Tehran, IRAN