Iranian Journal of Virology
مجله ویروس شناسی ایران
Iran J Virol
Medical Sciences
http://journal.isv.org.ir
1
admin
1735-5680
2588-5030
10.21859/isv
en
jalali
1397
9
1
gregorian
2018
12
1
12
2
online
1
fulltext
en
Novel RFLP Method for Detection of Four Avian Infectious Bronchitis Virus Genotypes (Massachusetts, 793/B, Variant2, and QX)
تخصصي
Special
پژوهشي
Original article
<div><strong>Background and Aims:</strong> Infectious bronchitis (IB) is one of the significant economically critical poultry diseases distributed worldwide, including Iran. Different IB virus (IBV) genotypes are circulating in different geographical regions. Typing of IBV strains is useful for implementation of control measures and for understanding the epidemiology and evolution of IBV. The S1 gene sequencing is used for IBV genotyping. Massachusetts, 793/B, QX, and IS-14194 IBV genotypes currently exist in Iranian poultry farms. This study aims to design an RFLP-based method to do genotyping and confirm of mentioned IBV genotypes.<br>
<strong>Materials and Methods:</strong> After amplification of parts of the S1 gene (RT-PCR), the PCR products treated with four enzymes (SfoI: Massachusetts, BtsCI: 793/B, MspI: IS-1494, and NruI: QX) and finally visualized on agarose gel electrophoresis.<br>
<strong>Results:</strong> Results showed 100 percent of the specificity of the newly designed method (in comparison with sequencing).<br>
<strong>Conclusions: </strong>This method can be used for primary confirmation and fast screening of Massachusetts, 793/B, QX, and IS-14194 IBV genotypes and even in local laboratories without the need for sequencing.</div>
Avian Infectious Bronchitis, Iran, RFLP, Genotyping
19
24
http://journal.isv.org.ir/browse.php?a_code=A-10-120-9&slc_lang=en&sid=1
Vahid
Karimi
Vkarimi@ut.ac.ir
10031947532846006962
10031947532846006962
No
Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Arash
Ghalyanchilangeroudi
Arashghalyanchi@gmail.com
10031947532846006963
10031947532846006963
Yes
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Fatemeh
Gholami
jalalg77@yahoo.com
10031947532846006964
10031947532846006964
No
Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Masoud
Hasehmzadeh
hashemzadehma@gmail.com
10031947532846006965
10031947532846006965
No
RAZI Institute, Karaj, Iran
AmirHossein
Mohseni
amirhossein_mohseni@hotmail.com
10031947532846006966
10031947532846006966
No
Department of Microbiology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
Hossein
Hosseini
hosseini.ho@gmail.com
10031947532846006967
10031947532846006967
No
Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Alborz, Iran
Naser
Sadri
naser.sadri@ymail.com
10031947532846006968
10031947532846006968
No
Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Amir
Modiri Hamdan
10031947532846006969
10031947532846006969
No
Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran