Iranian Journal of Virology
مجله ویروس شناسی ایران
Iran J Virol
Medical Sciences
http://journal.isv.org.ir
1
admin
1735-5680
2588-5030
10.21859/isv
en
jalali
1399
9
1
gregorian
2020
12
1
14
2
online
1
fulltext
en
Human Reovirus Serotype 3 Effectively Targets Huh-7 Cells
عمومى
General
پژوهشي
Original article
Background and Aims: Huh-7 is a cell line that was derived from a liver tumor of a Japanese man. Hepatocellular carcinoma (HCC) is considered as a primary liver cancer. Highly resistant tumor to treatment which causes the death of many patients annually. Thus, targeting the cancer cells by using a new method could be effective in therapy of this cancer. Reoviruses are oncolytic viruses that can infect and kill tumor cells, which have an activated Ras signaling pathways, while normal cells are resistant to infection and replication of these viruses. The aim of this study was to evaluate the effect of oncolytic human reovirus on Huh 7 cell line in vitro. Materials and Methods: Human reovirus serotype 3, Huh-7 cell line, and normal human fibroblasts were used in this study. After virus purification and plaque assay, human reoviruses were inoculated into the Huh-7 cells and human normal fibroblasts as negative control. Virus cytopathic effect, cell viability, and viral RNA replication were assessed at the different time of post-infection. Results: Virus cytopathic effects and cell lysis were clearly observed and reovirus RNA replication was detected in the Huh-7 cells, whereas normal human fibroblasts were resistant against reovirus infection. Conclusion: The result of the present study showed that human reoviruses serotype 3 can destroy the Huh-7 cells. Accordingly, the use of human reovirus could be considered as a potential therapy for HCC and liver cancer.
hepatocellular carcinoma (HCC), Huh-7, oncolytic virus, reovirus
23
28
http://journal.isv.org.ir/browse.php?a_code=A-10-234-2&slc_lang=en&sid=1
Reihaneh
Kazemi
re.kazemi71@gmail.com
10031947532846007166
10031947532846007166
No
Hepatitis & AIDS department, Pasteur institute of Iran, Tehran, Iran.
Angila
Ataei-Pirkooh
ataei.a@iums.ac.ir
10031947532846007167
10031947532846007167
No
Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Mohammad Reza
Aghasadeghi
mrasadeghi@pasteur.ac.ir
10031947532846007168
10031947532846007168
No
Hepatitis & AIDS department, Pasteur institute of Iran, Tehran, Iran.
Mohammad Hossein
Modaresi
Modaresi@tums.ac.ir
10031947532846007169
10031947532846007169
No
Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
Mojtaba
Hamidi-Fard
mojtaba_hamidifard@yahoo.com
10031947532846007170
10031947532846007170
Yes
Hepatitis & AIDS department, Pasteur institute of Iran, Tehran, Iran.