Iranian Journal of Virology

en Design and Fabrication of Rapid Diagnostic Test Based on Gold Nanosensors to Detect SARS-CoV-2 تخصصي Special پژوهشي Original article <div style="border-bottom:solid windowtext 1.0pt; border-top:solid windowtext 1.0pt; border-left:none; border-right:none; padding:1.0pt 0in 1.0pt 0in">Abstract</div> <div style="border-bottom:solid windowtext 1.0pt; border-top:solid windowtext 1.0pt; border-left:none; border-right:none; padding:1.0pt 0in 1.0pt 0in">Background and Aims: Early detection of COVID-19 can decrease the motality rate. The diagnostic tools available so far have been based on a) detection of viral genes, b) detection of human antibodies and c) detection of viral antigens, among which detection of viral genes by RT-PCR is recognized as the most reliable method. In order to speed up the detection process and reduce the false negative results presented, it is necessary to design an accurate and fast test. Methods: A specific probe against N gene was designed and then gold nanoparticles were synthesized by citrate reduction method. The properties of the synthesized Nano systems were determined in terms of size, surface charge, morphology and accuracy of nanoparticle formation using dynamic light scattering device, zeta potential, transmission electron microscope and ultraviolet light spectroscopy. The designed probe was added to gold nanoparticles after activation. Negative and positive samples were taken from patients and added to nanoparticles containing probes. Results: Due to the free the sulfhydryl group and the high bond energy between the gold and this group, after the probe was added to the nanoparticles, change in color was observed in the nanoparticle solution. UV Spectrum and DLS results showed 4 nm red shift and change in size from 11 to 16 nm, respectively. In the presence of the SARS-CoV-2 RNA, AuNPs-probe would bound with RNA particles, resulting in a space barrier against salt particle on the other hand in negative samples, the salt bridge would be formed and agglomeration of AuNPs and a change in color would be observed by naked eye. </div> Biosensor, Colorimetric detection, Gold Nanoparticles, Rapid test, SARS-CoV-2 1 8 http://journal.isv.org.ir/browse.php?a_code=A-10-313-1&slc_lang=en&sid=1 Angila Ataei-Pirkooh anjilaataei@gmail.com 10031947532846007809 10031947532846007809 No Department of virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Omid Pourdakan omid.pourdakan@yahoo.com 10031947532846007810 10031947532846007810 No Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran Ahmad Tavakoli ahmad.tavakoli66@gmail.com 10031947532846007811 10031947532846007811 No Department of virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Alireza Ghasempour mraagh22@gmail.com 10031947532846007812 10031947532846007812 No Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran Vahid Pirhajati-Mahabadi pirhajati.v@iums.ac.ir 10031947532846007813 10031947532846007813 No Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran Alireza Mohebbi 10031947532846007814 10031947532846007814 No Department of virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Bita Mehravi mehravibita@gmail.com 10031947532846007815 10031947532846007815 Yes Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran